人乳腺癌裸鼠异种移植的研究

A study on human breast carcinoma xenografts in nude mice

目的 研究人乳腺癌异种移植的自发转移、遗传稳定性和微转移的检测方法。方法 将人乳腺癌新鲜完整组织移植于裸鼠并连续传代 ,移植瘤细胞体外培养、再移植裸鼠并在 3个微卫星位点分析人乳腺癌、裸鼠移植瘤及其转移灶的微卫星DNA。结果 原位移植的成瘤率为 88.6 % (31/35 )、转移率为 41.9% (13/ 31)。移植瘤细胞体外培养成功 ,其再移植裸鼠成瘤率和转移率均达 10 0 %。裸鼠移植瘤及其转移灶在 3个位点与人乳腺癌具有相同的微卫星DNA。结论 完整组织原位移植法可提高人乳腺癌异种移植的成功率和转移率。异种移植瘤及其传代肿瘤保持了遗传稳定性。

Objective To study the spontaneous metastasis and the genetic stability of human breast carcinoma xenografts in nude mice and its micrometastasis. Methods The intact tissue of surgical specimens from breast carcinoma were xenografted into nude mice and transplanted from generation to generation. Cells from the xenografts were cultured in vitro and retransplanted into nude mice. Microsatellite DNA in genome of human breast carcinoma, xenotransplanted tumors and metastases in nude mice were analysed at three microsatellite loci. Results The tumorigenicity of orthotopic xenotransplantation was 88.6%(31/35) with a metastasis rate of 41.9%(13/31). Cells from the xenografts were cultured in vitro successfully, both the take rate of retransplantation into nude mice and the spontaneous lung metastasis rate were 100%(10/10). Microsatellite DNA sequences in genome of the xenotransplanted tumors and metastases in nude mice were identical with that of the original human breast carcinoma at the three microsatellite loci. Conclusions The tumorigenicity and metastasis potential can be improved in human breast carcinoma xenograft using the intact fresh tumor tissue and orthotopic graft. The xenotransplanted tumors and their passaged tumors maintained the genetic stability.The detection of microsatellite DNA may discover micrometastasis in the nude mice model.