银杏叶提取物、肌酸和氨哮素延缓去神经骨骼肌萎缩的实验研究

Effect of extracts of Ginkgo biloba, creatine and clenbuterol on retarding denervated skeletal muscular atrophy

目的 研究银杏叶提取物 (extractsofGinkgobiloba ,EGb76 1)、肌酸和氨哮素延缓去神经骨骼肌萎缩的效果及其机制。 方法 复制大鼠臂丛神经损伤动物模型 ,2 4只SD大鼠随机分为EGb76 1组、肌酸组、氨哮素组和对照组 ,分别以相应药物 ( 10 0mg·kg 1·d 1)及等渗盐水灌胃 ,5周后检测各组臂围、肌湿重、肌肉总蛋白含量 ,用末端转移酶介导的dUTP缺口末端标记法 (TdT -me diateddUTPnickendlabeling,TUNEL)检测萎缩肌肉中的细胞凋亡 ,用免疫组织化学染色及灰度分析检测肌细胞中fas、FLICE/Caspase8表达的变化。 结果 EGb76 1、肌酸和氨哮素不同程度地抑制去神经骨骼肌萎缩大鼠的臂围、肌肉湿重和肌肉总蛋白含量的降低 (P <0 .0 5 ) ,减少萎缩肌肉中的TUNEL染色阳性细胞数以及细胞中的fas、FLICE/Caspase8含量 (P <0 .0 1)。 结论 EGb76 1、肌酸和氨哮素能够有效地延缓去神经骨骼肌萎缩 ,其机制可能与抑制肌细胞凋亡有关。

Objective To study effect and mechanism of extracts of Ginkgo biloba (EGb), creatine and clenbuterol on retarding denervated skeletal muscular atrophy. Methods The animal model of brachial plexus injury was established in 24 rats which were randomly and equally divided into EGb group, creatine group, clenbuterol group and control group. Each group was administrated with EGb, creatine, clenbuterol and saline respectively with a dosage of 100 mg·kg 1 ·d 1 for 5 weeks. The circumference change of forelimbs, muscle wet weight and total amount of protein were measured. The apoptotic cells in atrophic muscle were measured with TUNEL, changes of fas and Caspase8/FLICE in muscle cells were also detected with immunohistochemistry. Results After administration of EGb, creatine and clenbuterol for 5 weeks, decrease of fore-limb, muscle wet weight and total amount of protein were inhibited ( P <0.05); and apoptotic cells, expression of fas and Caspase8/FLICE in atrophic muscle were decreased ( P <0.01). Conclusions EGb, creatine and clenbuterol can effectively retard denervated skeletal muscular atrophy. The mechanism may be related with inhibition of muscle cell apoptosis.