芳香烃和母亲某些代谢酶基因多态性对新生儿出生身长的影响

Association between genetic polymorphisms of microsomal epoxide hydrolase, the glutathione S-transferases in mother and infant birthweight

目的 :探讨芳香烃溶剂暴露和母亲细胞色素P45 0氧化酶 (MSP1) ,谷胱甘肽S转移酶Theta(GSTT1)和谷胱甘肽S转移酶M 1(GSTM1)的基因多态性对新生儿出生身长的影响。方法 :采用回顾性流行病学调查方法 ,使用统一调查表 ,由经过培训的调查员在北京燕山地区调查了 6 44个母亲婴儿对。结果 :(1)单因素分析发现 :GSTM1缺失基因型可致新生儿出生身长显著降低 ,但未观察到基因GSTT1、MSP1及芳香烃溶剂暴露对新生儿出生身长的影响。 (2 )采用多元线性回归模型 ,经母亲文化程度、年龄、被动吸烟、倒班、生育史、孕前身高、孕前体重、父亲身高、体重、婴儿性别、孕周、出生体重调整后 ,可见基因GSTM1、GSTT1及芳香烃溶剂暴露均能显著致新生儿出生身长降低。 (3)我们将芳香烃溶剂 (暴露与非暴露 ) ,母亲基因MSP1(野生型、杂合子 /突变型 )与基因GSTM1(存在型 ,缺失型 )分为 8组 ,在芳香烃溶剂暴露组 ,可见母亲基因型致出生身长呈斜线降低 ,然而在芳香烃溶剂非暴露组 ,其母亲基因型对出生身长的影响无明显改变。在芳香烃溶剂 (暴露与非暴露 ) ,MSP1杂合子 /突变基因型合并GSTT1缺失基因型组 ,我们得到了类似的结果。结论 :基因MSPI ,GSTT1,GSTM 1与芳香烃溶剂暴露之间对新生儿出生身长的影响可能存在交互作用 。

Objective: To investigate association between genetic polymorphisms of Cytochrome P450 1A1(MSP1), the glutathione S transferases (GSTM1 and GSTT1) in mothers' exposure to organic solvents and infants' birth lengths. Methods: This analysis was based on 744 singleton live born infant mother pairs in Beijing, China, with mothers neither active smoking nor drinking. Results: GSTT1(absent) was significantly associated with reduced birth length, but MSP1, GSTM1 polymorphisms and organic solvents exposure were not significantly associated with reduced birth length, respectively. After adjustment for major confounders including maternal age, education, parity, occupation, passive smoking, pre pregnant BMI, infant gestational age and sex, GSTT1, GSTM1 and organic solvents exposure were significantly associated with reduced birth length, respectively. Moreover, in comparison of birth lengths in eight subgroups defined by organic solvent status (nonexposure/exposure) and maternal genotype for MSP1 (AA, Aa/aa) and GSTM1 (present, absent), in the presence of organic solvents' exposure, the birth length was a clear gradient of adverseeffects. Howe ver, In the absence of organic solvents' exposure, the birth lengths did not differ significantly by maternal genotype. The similar results were observed in the group of organic solvent status(no, yes), MSP1(AA, Aa/aa) and GSTT1(present, absent) Conclusion: This study has provided strong evidence of gene environment interactions and offered a new research model to evaluate reproductive toxin in relation to adverse reproductive outcome.