摘要
以类离子液体碳糊电极（CILE）为基体电极，采用滴涂法和利用静电吸附作用，制备了Hb／Fe3O4／CILE修饰电极，研究了Hb的直接电化学及其电催化行为，建立了H20：的计时安培测定新方法。结果表明：Hb在该修饰电极上。Hb呈现了一对准可逆的氧化还原峰，且其在该修饰电极表面表观覆盖度为2．65×10-9moL／cm2；电子转移速率常数为1．35／s；表观米氏常数为1．59×10-5mol／L。在1．0×10-6～4．0×10-5mol／L范围内，催化电流与H202浓度呈线性关系（r=0．9976），检出限为3．0×10-7moL／L（S／N=3）。
Based on ionic liquid carbon paste electrode （CILE）, Hb/Fe304/CILE modified electrode was prepared by casting and static adsorption. The direct electrochemistry and electrocatalysis of Hb were investigated on the Hb/Fe304/CILE, and a new H202 determination method was established by chronoamperometry. The experimental results showed that a pair of quasi-reversible redox peaks of Hb was exhibited on the modified electrode. The average surface coverage of hemoglobin on the Hb/Fe304/CILE, the electronic transfer rate constant, and the apparent Michaelis-Menten constant were 2. 65 × 10-9mol/cm2, 1.35/s and 1.59× 10-5 mol/L, respectively. The proposed electrode exhibited good electrocatalysis towards H202, and the catalytic current was linear with concentration of H202in the range of 1.0 × 10-6 -4.0 × 10-5mol/L （r =0. 9976） with a detection limit of 3.0 × 10 -7 mol/L （ S/N = 3 ）.
出处
《分析试验室》
CAS
CSCD
北大核心
2013年第9期20-23,共4页
Chinese Journal of Analysis Laboratory
基金
国家自然科学基金项目(21275116)资助
