铜(Ⅱ)络合萃取FAAS法间接测定布洛芬

Indirect determination of ibuprofen by Cu^(2+) complexation extraction and FAAS

在pH 5.80的HAc-NaAc溶液中,布洛芬与Cu2+结合生成2:1难溶于水的络合物,经乙酸乙酯萃取分离后,通过火焰原子吸收光谱法测定下层清液中剩余的Cu2+,发现其吸光度A随药物浓度增加而减小。据此建立了Cu2+络合萃取FAAS法间接测定布洛芬新方法,并对方法的最佳测定条件进行了一系列的研究。在最佳条件下空白液与萃取后下层清液Cu2+的吸光度差△A和药物质量浓度在20.6~103μg/mL呈现良好的线性关系,检出限为15.3μg/mL,RSD为2.2%,用于布洛芬药片样品的测定,回收率在97.6%~101.2%。

Under the conditions of acetic acid-sodium acetate buffer solution at pH of 5.80, ibuprofen can react with Cu2＋ to form the complex of 2：1 ratio, which was insoluble in water. After extracted by ethyl acetate, the remainder Cu2＋in the inorganic phase was determined by FAAS. It was found that the absorbanee A decreased with the increase of drug concentration. Accordingly, a new method of simple, rapid indirect determination for ibuprofen by Cu2＋ complexation extraction and FAAS was established, and a series of the optimized measurement conditions were studied. The experiment results showed that there was a good linear relationship between ΔA, the absorbance difference of reagent blank and raffinate, and the concentration of ibuprofen in the range from 20. 6μg/mL to 103μg,/mL. The determination limit of this method was 15.3μg/mL, and the RSD was 2.2%. It has been successfully used for the determination of ibuprofen samples with satisfactory results, and the recoveries were between 97.6% and 101.2%.