含神经生长因子的几丁质管桥接兔面神经缺损的实验研究

EXPERIMENTAL STUDIES ON RABBIT FACIAL NERVE REGENERATION IN CHITIN TUBES CONTAINING NERVE GROWTH FACTOR

目的 探讨含神经生长因子 (NGF)的几丁质管在修复兔面神经缺损中的作用。方法 将 16只新西兰兔的两侧面神经上颊支分别造成 8mm的缺损 ,左侧用管腔内注入 NGF的几丁质管修复 ,右侧用自体神经移植修复作对照。术后 8、16周 ,分别取 8只兔进行电生理和超微结构研究。结果 实验侧术后 8周 ,再生神经中有髓和无髓神经纤维排列整齐 ,有髓神经的髓鞘厚 ,板层结构清晰 ;术后 16周 ,再生神经中有髓和无髓神经纤维数量增加 ,形态接近正常神经。实验侧和对照侧的神经传导速度无显著差异 (P>0 .0 5 )。结论 含 NGF的几丁质管可为兔面神经缺损提供良好的修复环境。

Objective To evaluate the nerve regeneration after implantation of chitin tubes containing nerve growth factor(NGF) in the rabbit facial nerve.Methods Bilateral 8 mm defect of superior buccal divisions of the facial nerves were made in 16 New Zealand rabbits. Chitin tubes containing NGF were implanted into the gaps, and autologous nerves were implanted into the right gaps as control. The nerve regeneration was evaluated with electrophysiological and ultrastructural examination after 8 and 16 weeks of operation.Results Chitin tubes containing NGF successfully induced the nerve regeneration, regularly arranged myelinated and unmyelinated axons could be observed across the 8 mm gaps, and the myelin sheath was thick with clear lamellar structure at 8 weeks after operation, The regenerated nerve fibers increased and were more mature at 16 weeks after operation. There were no significant difference in electrical impulse conduction velocity through the neural regeneration between the experimental and control sides (P>0.05). Conclusion Chitin tubes containing NGF can provide optimal conditions for regeneration of rabbit facial nerve.