Ku70和Ku80基因在系统性红斑狼疮患者皮肤上的表达

Expression of Ku70 and Ku80 genes in the skin of patients with systemic lupus erythematosus

目的 :研究Ku70和Ku80基因在系统性红斑狼疮 (systemiclupuserythematosus,SLE)患者皮肤表达的变化及其与SLE发病的关系。方法 :利用Ku70、Ku80地高辛标记的cDNAs探针对皮损区、非皮损区及正常对照皮肤进行原位杂交。结果 :Ku70、Ku80mRNAs的表达量从高到低依次为SLE皮损区、SLE非皮损区、正常皮肤。另外还发现在皮肤的层次上真皮浅层单一核细胞Ku70、Ku80mRNAs表达量高于表皮基底层细胞、表皮棘细胞层和颗粒层细胞。结论 :Ku70、Ku80mRNAs的表达在SLE患者皮肤上发病区高于非发病区 ,更大于正常皮肤。Ku70、Ku80mRNAs表达的变化与SLE的发病有关 。

Objective:To investigate the expression of Ku70 and Ku80 genes in the skin lesions of patients with systemic lupus erythematosus and its relationship with the pathogenesis of SLE. Methods:Ku70 and Ku80 mRNAs were detected in the lesional and non lesional skin of SLE patients and the skin of normal control by in situ hybridization with Dig labeled cDNA probes. Results: The abundance expression levels of Ku70 and Ku80 mRNAs were as following: the Ku70 、Ku80 mRNAs of SLE lesion is the highest, the SLE non lesional skin is in the middle, and the control skin is the lowest. Meanwhile the expression of Ku70 and Ku80 mRNAs in the dermal mononuclear cells(MNCs) was found to be stronger than that of basal layer keratinocytes and outside epidermis keratinocytes. Conclusion: The abundance of expression of Ku70 and Ku80 mRNAs in the skin area of SLE patients was significantly stronger than that of the control. Expression of Ku70 and Ku80 mRNAs is much related to the pathogenesis of SLE, the mechanism of which remains to be studied.