自体细胞因子诱导的杀伤细胞治疗急性白血病的临床研究

A pilot report of the effect of auto cytokine induced killers on the patients with acute leukemia

目的 :评价细胞因子诱导的杀伤细胞 (cytokineinducedkiller ,CIK)治疗急性白血病患者的有效性及安全性。方法 :采用血细胞分离机大量采集患者的外周血单个核细胞 ,再用抗CD3单抗、白细胞介素 2、干扰素γ培养10d左右 ,将细胞洗涤后经静脉回输给患者。结果 :37例急性白血病患者共接受 5 8疗程CIK治疗。 34例血液学缓解期接受CIK治疗患者的 3年持续完全缓解 (continuouscompleteremission ,CCR)概率为 75 % ,CIK治疗后的中位CCR期为 2 6月 (1～ 5 9月 )。经 1疗程CIK治疗后 ,2例 (2 /3)肿大的脾明显缩小 ,均CCR至今 (12 ,16月 ) ;8例(8/8)骨髓残留的异常染色体和 /或基因标志消失。CIK输注后除一过性发热 ,畏寒、疲乏外 ,无其它严重副作用。结论 :自体CIK细胞治疗具有明显清除微小残留白血病细胞、防止复发的作用 。

Objective: To evaluate the safety and therapeutic effects of auto cytokine induced killers (CIKs) on the patients with leukemia. Methods: The patients' peripheral blood mononuclear cells were collected with blood cell separator, then cultured in the medium containing monoclonal antibody against CD3, interleukine 2 and interferonγ for about 10 days in vitro , and infused back to the patients after washed twice. Results: 37 cases received total 58 courses of CIKs. The 3 years' CCR(continuous complete remission) probability after CIKs' therapy was 75% in 34 cases in hematological remission when the CIKs were infused, with the medium CCR period 26 months (1～59 months). After only 1 course of therapy, the enlarged spleen in 2 of 3 cases obviously shrank and had CCR (12,16 months); the residual abnormal chromosome and/or gene from 8 cases (8/8) disappeared in all the cases. Except transient fever, chill, fatigue etc, no remarkable side effects happened during or after auto CIKs' infusion. Conclusion: Intravenous infusion of auto CIKs can clear out minimal residual leukemic disease, prevent relapse and is demonstrated to be safe.