尼古丁对牙乳头间充质细胞增殖的抑制作用

The inhibitory effect of nicotine on the proliferation of human dental papilla mesenchymal cells in vitro

目的 :探讨尼古丁对牙乳头间充质细胞增殖的可能影响。方法 :用不同浓度的尼古丁硫酸盐作用于牙乳头间充质细胞 ,分别在 3、5、7d时 ,采用 MTT法观察细胞增殖情况。结果 :浓度低于 0 .0 5 g/L 时 ,A值变化不明显 (P>0 .0 5 ) ;当浓度高于 0 .1g/L 时 ,A值随尼古丁浓度增加而明显降低 (P<0 .0 1)。加药 1d时 ,实验组和对照组无明显差异 ,3d后出现差异 (P<0 .0 5 ) ,5～ 7d时实验组与对照组出现明显差异 (P<0 .0 1)。结论 :尼古丁抑制了牙乳头间充质细胞的增殖 。

Objective: To study the effect of nicotine on dental papilla mesenchymal cells. Methods: The effect of nicotine sulfate at various concentrations on the proliferation of human dental papilla mesenchymal cells was studied with MTT method. Results: When the concentration of nicotine sulfate was lower than 0.05 g/L,the absorbance value (A) of the treated cells was similar to that of the control( P >0.05).When the concentration of nicotine was higher than 0.1 g/L,the absorbance value of the treated cells was smaller than that of the control ( P <0.01),and the absorbance value decreased with the increace of nicotine dosage.In one day expossure group,no difference of absorbance values was found between the treated and the control cells,in three day and seven day expossure groups,the absorbance values of the treated cells were smaller than that of the control ( P <0.05 and P <0.01 respectively).Conclusion: Nicotine inhibite the proliferation of human dental papilla mesenchymal cells in a dose and time dependent manner.