黄芪皂甙对人系膜细胞基质分泌和β1整合素表达的影响

Effect of Astragalin on matrix secretion and β1 Integrin mRNA expression in human mesangial cells

目的了解黄芪皂甙对人肾小球系膜细胞（ＨＭＣ）的影响。方法应用不同浓度的黄芪皂甙或黄芪皂甙血清处理经人重组血小板衍化生长因子（ＰＤＧＦ－ＢＢ）刺激的培养的人系膜细胞，分别采用四甲基偶氮唑蓝（ＭＴＴ）法和酶联免疫吸附（ＥＬＩＳＡ）方法检测系膜细胞的增殖和Ⅳ型胶原分泌情况，用逆转录－聚合酶链反应（ＲＴ－ＰＣＲ）法检测β１整合素基因的表达。结果经黄芪皂甙或黄芪血清处理７２ ｈ后，由 ＰＤＧＦ－ＢＢ诱导的系膜细胞的增殖与未经处理的对照组相比呈显著抑制，并呈剂量依赖效应（Ｐ＜０．０５）；黄芪皂甙或黄芪血清作用２４ ｈ后，经ＰＤＧＦ－ＢＢ诱导的系膜细胞分泌的Ⅳ型胶原蛋白浓度和β１整合素ｍＲＮＡ水平均较未经用药的对照组有显著下降，并与剂量呈一定相关（Ｐ＜０．０５和Ｐ＜０． ０１）；β１整合素ｍＲＮＡ表达下调与人肾小球系膜细胞增殖抑制和Ⅳ型胶原蛋白浓度分泌下降之间存在显著性相关（Ｐ＜０．０５和Ｐ＜０．０１）。结论黄芪皂甙可抑制人肾小球系膜细胞增殖，抑制基质的过度生成和下调细胞表面β１整合素ｍＲＮＡ的表达。

Objective To investigate the effect of Astragalin on human renal mesangial cells. Methods Cultured human mesangial cells were treated with Astragalin and Astragalin serum in different concenrations in the presence of PDGF-BB,the proliferation and type Ⅳ collagen secretion of mesangial cells were measured by MTT assay and ELISA, and expression of β1 integrin gene was estimated by RT-PCR method, respectively. Results After 72 hours of Astragalin or Astragalin serum treatment, the proliferation of mesangial cells induced by PDGF-BB was inhibited significantly in a dose-dependent manner compared with untreated control(P < 0. 05 ). After 24 hours of Astragalin or Astragalin serum treatment, the secretion of type Ⅳ collagen protein in the presence of PDGF-BB was significantly decreased and β1 integrin mRNA level decreased significantly compared with untreated control(P < 0. 05 and P < 0. 01 ). Down-regulation of β1 integrin mRNA expression was significantly correlated with inhibition of mesangial cell proliferation and decrease of the type Ⅳ collagen protein secretion(P < 0. 05 and P < 0. 01 ). Conclusion Astragalin can inhibit proliferation and matrix over-synthesis of HMC, and down-regulate expression of β1 integrin on surface of HMC.