日本血吸虫细胞凋亡及其诱导的研究

STUDIES ON APOPTOSIS AND ITS INDUCTION IN SCHISTOSOMA JAPONICUM

[目的 ]探讨日本血吸虫的细胞凋亡现象及诱导日本血吸虫细胞凋亡的可能性。 [方法 ]分离、培养日本血吸虫成虫混合细胞和虫卵。用原位末端标记法和琼脂糖凝胶电泳法分别检测正常培养条件下和诱导因素存在时细胞凋亡发生情况。 [结果 ]未经诱导的成虫细胞 ,DNA琼脂糖凝胶电泳未见凋亡所特有的“梯形”条带 ,成虫细胞核可见少量明显的凋亡所特有阳性着色。经诱导的成虫细胞和卵细胞琼脂糖凝胶电泳均呈凋亡所特有的“梯形”条带 ,诱导组的成虫细胞核可见明显的凋亡所特有的阳性着色和凋亡小体。 [结论 ]首次观察到日本血吸虫成虫细胞存在凋亡现象 ,且外源性因素可诱发日本血吸虫成虫细胞和卵细胞凋亡。

Objective] To explore the apoptosis and its induction in S.japonicum. [Methods] The eggs and adult cells of S. japonicum were isolated. The adult cells were incubated with H 2O 2, dexamethasone and cyclosporin A, cyclosporin A vehicle solution, respectively in order to initiate the apoptosis. The eggs were treated at 4℃, 37℃ and 42℃, respectively for induction of apoptosis.The occurrence of apoptosis was supervised by agarose gel electrophoresis and in situ end labelling. [Results] DNA ladder band characteristic of apoptosis appeared in the cells of adult worms after incubated with H 2O 2, dexamethasone or cyclosporin A and the eggs treated at 42℃, whereas no DNA ladder band was found in other control groups. In situ end labeling revealed that the positive fluorescence could be seen in the cells incubated with H 2O 2, dexamethasone or cyclosporin A and few nuclei in the control group and cyclosporin A vehicle solution group appeared positive fluorescence. [Conclusion] Apoptosis in the cells of S.japonicum adults and eggs induced by chemical or physical agents was observed.