伯氏疟原虫氯喹抗性株感染小鼠的氯喹代谢动力学研究

STUDIES ON PHARMACOKINETICS OF CHLOROQUINE IN MICE INFECTED WITH CHLOROQUINE-RESISTANT STRAIN OF PLASMODIUM BERGHEI

[目的 ]比较氯喹在正常小鼠、感染伯氏疟原虫药物敏感 (N)株和氯喹抗药性 (RC)株小鼠体内的药物动力学差异。 [方法 ]应用反相高效液相色谱法分别测定正常小鼠、感染伯氏疟原虫N株和RC株小鼠血浆中的氯喹浓度 ,采用 3P87药物动力学分析软件对数据进行分析 ,从而获得有关药物动力学参数。 [结果 ]感染RC鼠的t1/2 β与其他两组间有显著的统计学差异 (P <0 .0 5 ) ,而感染N株鼠与正常鼠间无显著差异。 [结论 ]氯喹在感染了伯氏疟原虫RC株鼠体内的消除速度显著快于正常鼠及感染N株鼠。

Objective] To compare the pharmacokinetic differences of chloroquine in normal mice and the mice infected with the N and the RC strains of Plasmodium berghei . [Methods] The concentrations of chloroquine in the plasma of normal mice and the mice infected with the N or the RC strains of P. berghei were analyzed by reverse phase HPLC. The pharmacokinetic parameters were measured with software 3P87. [Results] The t 1/2 β value was significantly lower in the mice infected with the RC strain than in normal mice and the mice infected with the N strain( P <0.05), however, there were no significant differences between the mice infected with the N strain and normal mice. [Conclusion] Elimination of chloroquine in the mice infected with the RC strain of P. berghei speed up significantly comparing with the mice infected with the N strain.