摘要
[目的 ]探讨环孢素A体外抗曼氏血吸虫超微病理改变。 [方法 ]MF1小鼠实验感染曼氏血吸虫 6wk后 ,经主动脉和门静脉灌注收集虫体。将虫体放入含有 2 0 μg/ml环孢素A的M199培养液中体外培养。用扫描电镜和透射电镜观察药物所致的虫体损害。 [结果 ]药物作用后 ,大多数雄虫皮层肿胀、表面出现大小不一的结节、皮层外膜破溃、皮棘脱落、合胞体极度破坏 ;个别雄虫皮层出现空泡 ;雌虫皮层极度空泡变及合胞体受损。 [结论 ]环孢素A具有直接抗曼氏血吸虫的作用 ,合胞体受损是药物作用的主要机制。
Objective] To study the ultra pathological changes of syncytium of Schistosoma mansoni after cyclosporin A (CsA) treatment. [Methods] MF1 mice were infected with Schistosoma mansoni cercariae. Six weeks later, the adult worms were recovered by portal vein perfusion. After the worms were exposed to CsA of 20 μg/ml for 24 h, the drug induced damage of the worm surface was observed by SEM and TEM. [Results] Incubation of male and female schistosomes with 20 μg/ml of CsA for 24 h resulted in disruption of the tegument and rupture of the spines. Progressive surface damage and swelling and vacuolization of the tegument led to eventual disruption of the syncytium. [Conclusion]The antischistosomal action of CsA is direct, the syncytium is the main site for CsA attack.
出处
《中国寄生虫学与寄生虫病杂志》
CAS
CSCD
北大核心
2000年第5期293-295,共3页
Chinese Journal of Parasitology and Parasitic Diseases
关键词
环孢素A
曼氏血吸虫
合胞体
超微结构
cyclosporin A, Schistosoma mansoni , syncytium, ultrastructure