2Kao PM, Hsu BM, Chen CT, et al. Identification and quantifica- tion of the Acanthamoeba species and genotypes from reser- voirs in Taiwan by molecular techniques[J]. Acta Trop, 2014, 132: 45-50.
3Marciano-Cabral F, Cabral G. Acanthamoeba spp. as agents of disease in humans[J]. Clin Microbiol Rev, 2003, 16(2): 273- 307.
4Amaral Zettler LA, Nerad TA , O'Kelly CJ, et a/. A molecular reassessment of the Leptomyxid amoebae [ J ]. Protist, 2000, 151 (3): 275-282.
5Stothard DR, Schroeder-Diedrich JM, Awwad MH, et ol. The evolutionary history of the genus Acantharrtoeba and the identification of eight new 18S rRNA gene sequence types[J].J Eukaryot Microbiol, 1988, 45(1): 45-54.
6Byers TJ, Akins RA, Maynard BJ, et al. Rapid growth of Acanthomoeba in defined media; induction of encystment by glucose-acetate starvation[J]. J Protozool, 1980, 27(2): 216- 219.
7Brown TJ, Cursons RT. Pathogenic free-living amoeba (PFLA) from frozen swimming areas in Oslo, Norway[J]. Scand J In- fect Dis, 1977, 9(3): 237-240.
8Thomas V, Herrera-Rimann K, Blanc DS, et al. Biodiversity of amoebae and amoeba-resisting bacteria in a hospital water network [J]. Appl Environ Microbiol, 2006, 72 (4): 2428- 2438.
9JM Schroeder, GC Booton, J Hay, et al. Use of subgenic 18S ribosomal DNA PCR and sequencing for genus and genotype identification of Acanthamoebae from humans with keratitis and from sewage sludge [J]. J Clin Mic~obiol, 2001, 39(5): 1903-1911.
10Visvesvara GS. Classification of Acanthamoeba[Jl. Rev Infect Dis, 1991, 13 Suppl 5: $369-372.