青蒿琥酯对黑素瘤A875细胞增殖和凋亡的影响及机制研究

Effect of artesunate on the proliferation and apoptosis of melanoma A875 cells

目的观察青蒿琥酯对人皮肤恶性黑素瘤A875细胞增殖和凋亡的影响，并探讨相关机制。方法体外培养A875细胞，分别给予不同浓度的青蒿琥酯（10～80μg／rnl）作用细胞48h，MTT法检测抑制率，流式细胞术检测凋亡率，Westernblot法检测Caspase-3、Caspase-8、Caspase-9的蛋白表述水平变化。结果青蒿琥酯对A875细胞有明显的生长抑制和诱导凋亡的作用（P〈0.01），呈剂量依赖性；随药物浓度的增高.Caspase-3、Caspase-8、Caspase-9的蛋白表达量逐渐升高（P〈0.05）。结论青蒿琥酯可以通过死亡受体途径和线粒体途径诱导A875细胞的凋亡，从而抑制细胞增殖。

Objective To investigate the influence of artesunate on the proliferation and apoptosis of human skin malignant melanoma A875 cells, and to explore its possible action mechanism. Methods The A875 cells were cultured with different concentration （10p, g/ml -80lμ/ml） of artesunate for 48 hours in vitro. The inhibition ratio was examined by MTr method, and apoptotic rate was detected by flow cytometry. The changes of protein expression of Caspase-3, Caspase-8 and Caspase-9 were detected by Western Blot. Results A875 cells proliferation was significantly inhibited by artesunate in a concentration-dependent manner. The apoptosis of A875 cells was accelerated in a concentration-dependent manner too. With the increase of drug concentration, the protein expression levels of Caspase-3, Caspase-8 and Caspase-9 were gradually increased. Conclusion Artesunate can induce apoptosis of A875 cells via death receptor pathway and mitochondrial pathway, as a result, artesunate has an obvious inhibition effect on A875 cells proliferation.