新型的分子印迹固相萃取小柱分离富集饲料中的三聚氰胺

Separation and Enrichment of Melamine in Feed by a New Molecular Imprinting Solid-phase Extraction Column

[目的]研制一种新型的分子印迹固相萃取小柱,用于分离富集饲料中的三聚氰胺。[方法]采用本体聚合法制备了三聚氰胺分子印迹聚合物,以该聚合物为填料制作三聚氰胺分子印迹固相萃取(MIP-SPE)小柱,优化了固相萃取条件,应用高效液相色谱(HPLC)法测定浓缩液中的三聚氰胺含量。[结果]自制的MIP-SPE小柱可以从饲料中选择性地分离、富集三聚氰胺,有效地去除饲料中的复杂基质,加标回收率约为95%。MIP-SPE小柱与空白印迹固相萃取小柱(NMIP-SPE)的对比试验表明,前者的萃取效率明显高于后者。[结论]自制的新型三聚氰胺MIP-SPE小柱可用于饲料提取液中的三聚氰胺高选择性分离富集,使用简便,具有广阔的应用前景。

[ Objective] The research aimed to develop a new molecular imprinting solid-phase extraction column for the separation and enrichment of melamine in feed. [ Method ] The molecular imprinting polymer of melamine was prepared by noumenon polymeric method. This polymer was used as filler to prepare molecular imprinting polymer solid-phase extraction（MIP-SPE） column of melamine. The solid-phase extraction conditions were optimized and melamine content in concentrated solution was determined by using HPLC. [Result] Melamine could be separated selectively from feed and enriched by home-made MIP-SPE column. It could wipe off complex matrix from feed and the addition recovery rate was 95%. The contrast experiment between MIP-SPE column and NMIP-SPE （non-imprinted polymer-solid phase extraction） column showed that the extraction efficiency of the former was obviously higher than the latter. [ Conclusion] The home-made MIP-SPE column could be used to separate and enrich melamine in feed efficiently,with a broad application prospect.