PCR-DGGE技术分析沼气生物脱硫系统中的优势菌种

Identification of the Superiority Strains in the Biological Desulfurization of Biogas by PCR-DGGE Method

文章采用PCR-DGGE技术分析某污水处理厂污泥厌氧消化沼气生物脱硫系统中的优势菌种。测得的16SrDNA序列在GenBank数据库进行检索和同源性分析。结果表明,反应器中有5种优势菌株:Halothiobacillus nea-politanus strain CIP 104769,Thiobacillus sp.ISA11,Sulfurimonas autotrophica DSM 16294,Sulfurimonas sp.RS_Sur15-1,Uncultured bacterium clone J69-151C-2A。结合DGGE谱图条带亮度,系统中优势脱硫细菌依次为:硫杆菌属的Thio-bacillus sp.ISA11,螺杆菌科Sulfurimona属的Sulfurimonas autotrophica DSM 16294,盐生硫杆菌属的Halothiobacillusneapolitanus strain CIP 104769,螺杆菌科Sulfurimona属的Sulfurimonas sp.RS_Sur15-1。

PCR-DGGE method was used to identify the superiority strains in the biological desulfurization of biogas for an- aerobic sludge digestion system. Homology analysis was conducted by comparing these gene sequences based on GenBank database. Results showed that the superiority strains in the system were HalothiobnciUus ncapolitanus strain CIP 104769, ThiobaciUus sp. ISAll, Sulfurimonas autotrophica DSM 16294, Sulfurimonas sp. RS_Surl5-1, Uncultured bacterium done J69-151C-2A. According to the light intensity of the bands on DGGE spectrogram, the superiority strains of desulfurization were as order as follows ： Thiobacillus sp. ISA11 ,Sulfurimonas autotrophica DSM 16294 ,Halothiobncillus neapolitanus strain CIP 104769 ,Sulfurimonas sp. RS_Sur15-1.