金雀花的薄层鉴别及黄酮类化合物的含量测定

On determination of flavonoids in the flower of caragana altaica kom.pojark by HPLC and thin-layer chromatography

目的研究金雀花的薄层色谱鉴别特征,建立HPLC法测定黄酮类化合物的方法。方法采用薄层色谱法对金雀花中槲皮素和山萘酚进行定性鉴别,槲皮素以甲苯-乙酸乙酯-甲酸=5∶2∶1为展开剂,山萘素以甲苯-乙酸乙酯-甲酸=5∶4∶1为展开剂;采用色谱柱：Shim-pack VP-ODS（150mm×4.6mm）,以甲醇-0.4%磷酸溶液（50∶50）为流动相,流速为1mL/min,检测波长为360nm,柱温为25℃。结果在薄层色谱中能检测到槲皮素和山萘酚,槲皮素、山萘素、异鼠李素分别在0.01~0.1、0.002 5~0.025、0.002 5~0.025mg/mL范围内线性良好,其平均回收率分别为100.7%、100.4%、101.7%。RSD分别为1.3%、1.4%和1.9%。样品黄酮类化合物的平均含量为1.25%。结论该方法简便、快速、准确、重复性好,可用于测定金雀花中黄酮类化合物的含量。

Objective To establish a HPLC method for the determination of flavonoids content incaragana altaica Kom.Pojark by the studies on TLC identification characteristics of Caragana flowers in Aletai.Methods Quercetin and kaempferol were identified by TLC,The HPLC analysis was performed on a Sunfire C18（150mm×4.6mm,5μm）column.a mixture of acetonitrile-0.4%phosphoric acid aqueous solution（50∶50）was used as the mobile phase with the flow rate at 1mL/min.The detection wavelength was set at 360nm.The column temperature was set at 25℃.Results Quercetin and kaempferol could be detected by TLC quercetin,kaempferol and isorhamnetin showed good linearity in the ranges of 0.010.10mg/mL,0.0025-0.025mg/mL and 0.0025-0.025mg/mL.The average recoveries were 100.7%,100.4%,101.7%.RSD1.3%,1.4% and 1.9%.Samples of flavonoid content averaged 1.3%.Conclusion The method is simple,rapid,accurate and reproducible and it can be used for the determination of the caragana altaica Kom.Pojark in the flavonoids content.