鳗弧菌感染牙鲆的组织学和免疫组织化学观察

HISTOPATHOLOGY AND IMMUNOHISTOCHEMISTRY OF FLOUNDER INFECTED BY Vibrio anguillarum

利用免疫组织化学ABC法及组织学HE染色技术对鳗弧菌人工感染后的牙鲆肝组织及胃肠道进行了组织病理学及细菌的定位研究。结果显示 ,感染后肝细胞肿胀 ,空泡化现象严重 ,肝窦扩张,肝组织内可见阳性反应的细菌呈堆状存在;胃黏膜褶变矮 ,胃壁变薄 ,黏膜层内可见阳性反应的细菌;肠绒毛细胞出现破损 ,肠腔内有大量细胞碎片,肠腔内也可见阳性反应的细菌。提示 :鳗弧菌可直接侵入组织细胞破坏组织结构 ,进而使鱼体出现死亡。

Liver and gastrointestinal tract of flounder infected by Vibrio anguillarum was studied using immunnhistochemical ABC method and HE staining . The results showed that hepatocytes were inflated, sinusoids were enlarged, stomach mucosa folds were weaken, stomach wall became thinner, intestinal villi cells were broken and intestinal lumen was filled with cell fragments. Immunoreactive positive bacterium clumps were detected in liver, stomach mucosa and intestinal lumen. These results indicated that V.anguillarum can directly invade tissues and cells to destroy their structure and last make fishes dead.