输液器污染微生物辐射抗性频率分布的研究

DISTRIBUTION OF RADIATION RESISTANT MICROORGANISM AND BIOBURDEN ON INFUSION SET

从 5 6套输液器分离得到 3 0 3 2株分离菌 ,系统地研究了输液器上污染微生物的辐射抗性。用辐射处理获得了较高抗辐射的微生物 98株 ,测定了它们的辐射抗性D10 。分离菌株的D10 是在 0 8～ 4 0kGy之间 ,主要集中在 1 2～ 1 6kGy。D10 ≤1 6kGy占全部污染菌的 99%。所有辐射筛选菌株是革兰氏阳性菌 ,其中 84株是Bacillussp .,1 4株是micrococcussp .。在输液器外壁获得了 1株桔红色的菌株 ,其D10 为 4 0kGy。

Radiation screened 98 isolates from 3032 cfu on 56 infusion sets were studied for radiation resistance. The D 10 values of these screened isolates were 0\^8 to 4\^0 kGy and mostly ranged within 1\^2 to 1\^6kGy. The microorganisms with D 10 ≤1\^6kGy were about 99 0% of the total bioburden. All of the screened isolates were gram\|positive bacteria, and 84 isolates were classified as Bacillus sp. and 14 isolates were characterized as Micrococcus sp.. One isolate with D 10 of 4\^0kGy (from D\|6=24kGy) was found outside of the infusion sets, accounting for 0\^033% of the total bioburden. The most resistant bacteria were orange colony and were classified as a Micrococcus sp. with broad shoulder.