缺血引起陷窝蛋白-1在肺泡Ⅱ型上皮细胞定位的改变

Ischemia Induced Caveolin-1 Moving from Cell Membrane to Lipid Droplets in Type Ⅱ Alveolar Epithelial Cell

肺泡Ⅱ型上皮细胞在肺缺血病理过程中具有重要作用。为研究缺血对陷窝蛋白-1在肺泡Ⅱ型上皮细胞A549脂滴定位的影响,利用已经建立的脂滴纯化方法,纯化得到肺泡Ⅱ型上皮细胞A549的脂滴,并在脂滴上发现了陷窝蛋白-1。在A549细胞缺血模型中发现缺血可以导致陷窝蛋白-1从细胞膜移动到脂滴。人肺组织脂滴纯化实验也证实缺血可以刺激陷窝蛋白-1从细胞膜移动到脂滴。这一发现将为肺缺血机制的研究提供新的思路。

Type II alveolar epithelial cells play an important role in ischemia of the lung. In this research, the authors studied the intracellular location of the caveolin-1 in type II alveolar epithelial cells under normal and ischemia status. They purified the lipid droplets from type II alveolar epithelial cell line A549. The results indicated that caveolin-1 was localized on plasma membrane as well as lipid droplets of alveolar epithelial cell, whereas ischemia stimulus induced caveolin-1 moving from cell membrane to lipid droplets in A549 cell line. In human lung tissue, They also observed the translocation of caveolin-1 from cell membrane to lipid droplets under ischemia status. These findings may promote new directions in future research concerning the mechanism of lung ischemia injury.