血管生成抑制剂TNP-470抑制腺样囊性癌转移的实验研究

An experimental study of angiogenesis inhibitor TNP-470 on the metastasis of the human salivary adenoid cystic carcinoma in vivo

目的 观察血管生成抑制剂TNP - 470对人涎腺腺样囊性癌肺转移的抑制作用。方法 将腺样囊性癌ACC -M细胞接种于裸鼠尾静脉 (1× 10 6/鼠 )。将 2 0只裸鼠随机分成对照组和治疗组 ,自第 2天起分别给予溶剂 (3%酒精 )和TNP - 470 (4 0mg/kg) ,隔天给药 1次 ,共用 4周。接种后 6周末处死动物 ,检测对照组和治疗组的肺转移率及含转移灶的肺重量 ,观察转移情况。结果 对照组和治疗组肺转移率分别为 90 %和 40 % (P <0 .0 2 5 ) ;含转移灶的肺重量分别为 0 .2 138g±0 .0 6 6 7g和0 .16 85 g±0 .0 44 5 g(P <0 .0 5 )。结论 血管生成抑制剂TNP -

Objective To study the effect of angiogenesis inhibitor TNP-470 on the metastasis of salivary adenoid cystic carcinoma in vivo.Methods Human salivary adenoid cystic carcinoma (Cell line ACC-M) cells (1×10 6/mouse) were injected into the tail vein of 20 nude mice,which were randomized into 2 groups.From the 2nd day,the treated group was given 40mg/kg of TNP-470 SC every other day for four weeks and the control group was given vehicle only (3% ethanol).At the 6th week after injection,the mice were sacrificed and the lung metastasis rates and the weight of the lung of the 2 groups were detected.Results The lung metastasis rate of the control and treated groups was 90% and 40% respectively (P< 0.025 ).The lung weight of the control and treated groups were 0.2138 g± 0.0667 g and 0.1685 g± 0.0445 g (P< 0.05 ) respectively.Conclusion These results suggest that the angiogenesis inhibitor TNP-470 has a strong inhibitory effect on metastasis of salivary adenoid cystic carcinoma.