白癜风患者CLEC2B mRNA差异表达与临床相关性研究

Differential expression of CLEC2B mRNA in vitiligo and its clinical implications

目的探讨CLEC2B（C—type lectin domain family 2，memberB）基因在白癜风发病中的作用。方法实时荧光定量PCR方法检测白癜风患者皮损区及外周血中CLEC2B mRNA表达，比较不同临床类型、病期、病程CLEC2BmRNA的差异表达。结果白癜风患者组皮损中，CLEC2BmRNA表达量高于健康对照组（两组表达量分别为1．21±0．03，1．00），差异有统计学意义（t=4．432，P〈0．05）。白癜风患者与健康对照组外周血CLEC2BmRNA表达量比较，差异无统计学意义（两组表达量分别为1．02±0．05，1．00；t=1．435，P〉0．05）。寻常型白癜风皮损组中，CLEC2BmRNA表达量高于节段型白癜风组（1．21±0．03，1．02±0．01），差异有统计学意义（t=5．330，P〈0．05）。不同病程白癜风患者皮损中，CLEC2BmRNA表达量比较，差异无统计学意义（P〉0．05）。进展期白癜风患者组皮损中CLEC2BmRNA表达量高于稳定期患者组（1．25±0．05比1．08±0．03），差异有统计学意义（t=3．046，P〈0．05）。结论CLEC2BmRNA的差异表达可能参与白癜风的发病。

Objective To evaluate the role of C-type lectin domain family 2, member B （CLEC2B） gene in the pathogenesis of vitiligo. Methods Real time fluorescence-based PCR was performed to detect the expression of CLEC2B mRNA in the peripheral blood and lesional skin of 37 patients with vitiligo as well as in the peripheral blood and normal skin of 40 healthy controls. Data were statistically analyzed by t test and chisquare test. Results Among the 37 patients, 23 had progressive vitiligo, 14 stable vitiligo, 31 vitiligo vulgaris, 6 segmental vitiligo. The expression level of CLEC2B mRNA was significantly higher in vitiligo lesions than in the control skin （1.21 ± 0.03 vs. 1.00, t = 4.432, P 〈 0.05）, but was of no significant difference in peripheral blood between the patients and healthy controls （ 1.02 ± 0.05 vs. 1.00, t = 1.435, P 〉 0.05）. Increased expression of CLEC2B mRNA was noted in lesions of vulgaris vitiligo compared with those of segmental vitiligo （ 1.21 ± 0.03 vs. 1.02 ± 0.01, t = 5.330, P 〈 0.05）, as well as in lesions of progressive vitiligo compared with those of stable vitiligo （1.25 ± 0.05 vs. 1.08 ± 0.03, t = 3.046, P 〈 0.05）. No significant difference was observed in the expression of CLEC2B mRNA among lesions of vitiligo with different courses （P 〉 0.05）. Conclusion The differential expression of CLEC2B mRNA may take part in the pathogenesis of vitiligo.