辣椒种质资源抗青枯病的鉴定与评价

Identification and Evaluation of Resistance to Bacterial Wilt in Pepper

采用青枯菌FJC100301菌株对田间辣椒(Capsicum annuum)抗病品种76a和感病品种TW-1分别作了不同温度、不同接种量和不同接种方法的接种试验。结果表明,辣椒青枯病抗性的室内鉴定以接种温度28℃、浸根20 min和3×108cfu/mL接种浓度为宜;辣椒种质田间抗青枯病接种鉴定宜选择5月上旬进行,浸根20 min,接种浓度为3×108cfu/mL。采用田间抗性接种鉴定的方法,用青枯菌FJC100301菌株对106份辣椒材料进行了抗性鉴定。田间接种后每隔10 d统计病情指数,划分辣椒抗青枯病鉴定分级标准,获得了高抗材料14份、抗病材料8份、中抗材料23份、中感材料23份、感病材料20份、高感材料18份;采用离体叶片接种法对田间筛选得到的高抗和高感纯度较高品种进行抗性分析,结果与田间鉴定一致。

Resistant levels of resistant pepper cultivar （76a） and susceptible pepper cultivar （TW-1） plants were evaluated after infection with Ralstonia solanacearum FJC100301 under different temperatures,concentrations and methods of inoculation.The results showed resistant identification of pepper plants grown in a growth room at 28℃ and infected with 3×108 cfu·mL-1 R.solanacearum suspension through root-soaking at 20 min.Resistant was identified and evaluated in pepper plants grown in the field after inoculation with 3×108 cfu·mL-1 R.solanacearum suspension through root-soaking at 20 min in early May. A total of 106 pepper cultivars were tested in the field after infection with R.solanacearum FJC100301. Disease indexes were scored every 10 day. Meanwhile,standards of resistant levels were divided in pepper. The results showed high resistance in 14 pepper cultivars,resistance in 8 pepper cultivars,moderate resistance in 23 pepper cultivars,moderate susceptibility in 23 pepper cultivars,susceptibility in 20 pepper cultivars,and high susceptibility in 18 pepper cultivars. The same results were obtained in several high resistance and high susceptibility pepper inbred cultivars through leaf inoculation with Ralstonia solanacearum in vitro.