子宫内膜样腺癌组织中p57^(KIP2)、cyclin E蛋白表达与临床病理特征的关系

Relationship between Expression of p57^(KIP2) and cyclin E Proteins and Clinicopathologic Features in Endometrial Adenocarcinoma Tissues

目的研究子宫内膜样腺癌组织中p57KIP2、cyclin E蛋白的表达与临床病理特征的关系。方法采用免疫组织化学Elivision法检测100例子宫内膜样腺癌(endometrioid adenocarcinoma,EA)、20例正常子宫内膜、20例子宫内膜增生性病变、20例子宫内膜上皮内瘤变(endometrial intraepithelial neoplasia,EIN)组织中p57KIP2、cyclin E蛋白的表达情况。结果 p57KIP2蛋白在EIN中阳性表达最高,在增生性病变中表达最低,在正常内膜组织中表达稍高于EA,但仅在EIN与在增生性病变中阳性表达比较,差异有统计学意义(P≤0.05);cyclin E蛋白在正常子宫内膜组织中表达最低,在增生性病变、EIN中的阳性表达逐渐增高,在EA中表达最高(P≤0.05,P<0.01)。在EA组织中,p57KIP2、cyclin E蛋白的阳性表达均与组织学分级、肌层浸润深度、手术分期有关(P≤0.05,P<0.01),cyclin E蛋白的表达还与盆腔淋巴结转移有关(P<0.01);但两者的阳性表达均与患者年龄无关(P>0.05)。p57KIP2与cyclin E之间的阳性表达呈负相关(P<0.01)。结论p57KIP2、cyclin E均参与了EA的发生发展过程。联合检测p57KIP2、cyclin E蛋白在子宫内膜癌组织中的表达情况,对评估子宫内膜癌的生物学行为、判断预后有重要的意义。

Objective To research the relationship between the expressions of p57^KIP2 and cyclin E proteins and clinical pathological features in endometrial adenocarcinoma. Methods The expressions of p57^KIP2 and cyclin E proteins in 100 cases of endometrial adenocarcinoma（EA）, 20 cases of normal endometrium tissues,20 cases of endometrial proliferative lesions and 20 cases of endometrial intraepithelial neoplasia （EIN） were detected respectively by immunohistochemical Elivision methods. Results The positive expression of p57^KIP2 protein was the highest in EIN,and lowest in proliferative lesions, and slightly higher in normal endometrial tissue than that in EA. But only between EIN and proliferative lesions, the difference of p57^KIP2 was significant（P≤0. 05）. The positive expression of cyclin E protein was the lowest in normal endometrial tissue, and gradually increased in proliferative lesions and EIN, and the highest in EA （P≤0. 05,P〈0. 01）. In EA, the expressions of p57^KIP2 and cyclin E proteins were associated with histological grading, depth of myometrial invasion and surgical stages （P≤0. 05, P〈0. 01）. The expression of cyclin E protein was associated with pelvic lymph node metastasis （P〈0. 01）. Both protein expressions were unrelated to age （P〉0. 05）. There was negative relationship between p57^KIP2 and cyclin E protein （P〈0. 01）. Conclusion p57^KIP2 and cychn E might be involved in the development and progression of endometrial carcinoma and predict the biological behavior of carcinoma.