氨基脲缓释给药孕鼠诱导建立新生鼠胸主动脉夹层模型

Establishment of neonatal rat model of thoracic aortic dissection by treating mother rats with semicarbazide

目的建立氨基脲诱导孕大鼠使其出生幼鼠形成胸主动脉夹层模型。方法分别将不同浓度[5、10、15、20、25、30、35、40mg/(kg·d)]氨基脲、生理盐水加载植入式胶囊渗透压泵,在孕大鼠妊娠期第14天时进行腹腔埋植,取妊娠期第21天的胎鼠及新生鼠的胸主动脉血管,常规H-E染色光镜下观察,统计并分析胸主动脉夹层形成情况。结果不同浓度氨基脲给药孕大鼠孕育的幼鼠在出生后均有不同程度的胸主动脉夹层出现,其中以缓释速率为每天25mg/kg氨基脲为最适给药浓度。此种浓度给药孕大鼠生产的新生鼠全部成活并且存在胸主动脉夹层现象。结论成功建立氨基脲缓释给药孕鼠诱导的新生大鼠胸主动脉夹层模型,为后续研究奠定了基础。

Objective To establish a neonatal rat model of thoracic aortic dissection by treating pregnant rats with semicarbazide. Methods Implantable capsule osmotic pump with different concentrations of semicarbazide （5, 10, 15, 20, 25, 30, 35, 40 mg/[kg · d]）or normal saline were implanted into the abdomen of rats on day 14 of pregnancy. The thoracic aortas of 21 day fetus and neonatal rats were collected. H-E staining was applied to examine the pathological changes under microscope, and the formation of thoracic aortic dissection was analyzed. Results Various degrees of thoracic aortic dissections were observed in neonatal rats after the mother rats were given different concentrations of semicarbazide. At a lowest semicarbazide release rate of 25 mg/kg per day, all the neonatal rats survived and had a high. incidence of thoracic aortic dissection. Conclusion A neonatal rat model of thoracic aortic dissection has been successfully established, paving a way for further researches.