川芎嗪调节Bcl-2促进帕金森病小鼠多巴胺神经元存活

Effect of tetramethy lpyrazine-administration on tyrosine hydroxylase in midbrain of Parkinson disease mice model

目的探讨川芎嗪对PD小鼠多巴胺神经元的保护作用。方法 30只健康雄性C57BL/6小鼠,随机分为正常对照组、模型组和治疗组。模型组和治疗组给予腹腔注射百草枯和代森锰建立PD模型,建模成功后,治疗组给予川芎嗪腹腔注射2 w,正常对照组给予同剂量生理盐水。观察3组小鼠行为学的改变、并用免疫组织化学、Western印迹法检测中脑多巴胺神经元数量、形态参数及Bcl-2的表达。结果模型组与治疗组比较,爬杆时间明显延长,中脑多巴胺神经元数量显著减少、Bcl-2的表达显著增高。结论川芎嗪治疗可上调Bcl-2的表达,增加黑质TH阳性神经元数量,有明显的神经保护作用。

Objective To investigate the effect of Tetramethy Lpyrazine on tyrosine hydroxylase（TH） in midbrain of Parkinson dis-ease（PD） mice model induced by paraquat and maneb. Methods Male C57BL/6 mices were randomly divided into PQ ＋ MN treated, Tet-ramethy Lpyrazine treated and control groups. Behavioral, immunohistochmical and western blot were used to detect the motor coordination and the expressions of TH, bcl-2 in midbrain. Results Administration of Tetramethy Lpyrazine could significantly attenuate the impairment of behavioral performance, increase the expression of TH and bcl-2 in midbrain. Conclusions Tetramethy Lpyrazine has a beneficial influ- ence on parkinsonism by increase bcl-2 in midbrain.