禽白血病病毒J亚群自然感染蛋鸡病毒负载、受体表达及致瘤谱关系

The Relationship of Virus Load,Receptor Expression and Tumor Spectrum in Layer Chickens Infected by ALV-J

J亚群禽白血病病毒(Subgroup J avian leukosis virus,ALV-J)通过与宿主细胞膜上的受体chNHE1蛋白结合感染细胞并引起宿主发病,进而引起肿瘤。本研究对确诊为ALV-J感染的肿瘤携带鸡进行病理组织学观察和病毒分离,应用荧光定量PCR技术检测ALV-J自然感染蛋鸡体内ALV-J负载量与其受体chNHE1蛋白mRNA表达量,分析ALV-J负载量、受体表达量及致瘤谱之间的相关性。结果显示:ALV-J在蛋鸡及地方品种鸡体内所诱发的肿瘤呈现多样化,但是病毒负载量与肿瘤谱相关性不明显;ALV-J负载量与chNHE1蛋白mRNA表达量呈正相关;组织在发生肿瘤时chNHE1蛋白mRNA表达量升高,可见,chNHE1蛋白不仅是ALV-J感染宿主细胞的受体,而且在肿瘤形成过程中也发挥重要作用。本研究为ALV-J致瘤机制的深入研究提供了科学基础。

Subgroup J avian leukosis virus （ALV-J） infect cells by binding to the chNHE1 receptor protein of the host and causes tumors. The tumor incidence of the ALV-J-infected chickens was observed by histo- pathology, and virus was isolated on DF-1 cell line. The ALV-J load and mRNA of chNHElreceptor pro- tein were detected by real time PCR. The relationship between ALV-J load, chNHE1 receptor expression levels and tumor spectrum was analyzed. The results showed that the tumors induced by ALV-J in laying hens and local lines of chicken were different. No significant relationship was observed between ALV-J load and tumor spectrum. ALV-J load was positively correlated with mRNA expression of cbNHE1. The mRNA expression of chNHE1 increased when the tumors occurred. Our results suggest the chNHE1 pro- tein is not only the receptor of ALV-J infected host but also play an important role in the process of tumor development. This study provides a scientific basis for further studying of oncogenic mechanism of ALV-J.