钝裂银莲花花粉活力测定方法的研究

Detection Methods for Pollen Viability of Anemone obtusiloba

以3种花色（黄色、浅黄色和白色）钝裂银莲花为研究对象，分别采用TFC染色、I2-KI染色、无机酸测定和蔗糖萌发4种方法，测定了钝裂银莲花（Anemone obtusiloba）的花粉活力，同时比较了不种方法对花粉活力的测定效果，并了解了花粉活力在一天内的动态变化情况。结果表明：（1）钝裂银莲花花粉可育率很高，达80％左右。花粉直径为28．75—33．75μm。（2）Trc浓度影响花粉活力的测定，当浓度为0．5％时，所测花粉活力的效果较好。（3）I2-KI法的染色效果不理想，染色率仅为8．33％-22．2％，且3种花色花粉活力测定数据间差异不显著。（4）3种花色钝裂银莲花花粉活力有差异，黄色最高，浅黄色次之，白色最低。TTC染色法是简单快速测定钝裂银莲花花粉活力的最适方法。一天内花粉活力在12：00～14：00最大。

The pollen viability of Anemone obtusiloba with three-color flowers （yellow, pale yellow and white） was detected with four methods： TYC dyeing, I2-KI dyeing, inorganic acid test, sucrose-based germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that： （ 1 ） The fertile rate of pollen of A. obtusiloba is high, about 80%. Pollens diameter are 28.75 - 33.75 p,m. （2） The concentration of TYC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. （3） I2-KI dyeing is not ideal, dyeing rate is only 8.33% -22.2%, and there is no significant difference within three-color flowers. （4） The pollen vitality of A. obtusiloba with three-color flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TYC dyeing is a simple and rapid determination method of A. obtusiloba. Within a day, pollen vitality is maximum from 12：00 - 14：00 pro.