摘要
以3种花色(黄色、浅黄色和白色)钝裂银莲花为研究对象,分别采用TFC染色、I2-KI染色、无机酸测定和蔗糖萌发4种方法,测定了钝裂银莲花(Anemone obtusiloba)的花粉活力,同时比较了不种方法对花粉活力的测定效果,并了解了花粉活力在一天内的动态变化情况。结果表明:(1)钝裂银莲花花粉可育率很高,达80%左右。花粉直径为28.75—33.75μm。(2)Trc浓度影响花粉活力的测定,当浓度为0.5%时,所测花粉活力的效果较好。(3)I2-KI法的染色效果不理想,染色率仅为8.33%-22.2%,且3种花色花粉活力测定数据间差异不显著。(4)3种花色钝裂银莲花花粉活力有差异,黄色最高,浅黄色次之,白色最低。TTC染色法是简单快速测定钝裂银莲花花粉活力的最适方法。一天内花粉活力在12:00~14:00最大。
The pollen viability of Anemone obtusiloba with three-color flowers (yellow, pale yellow and white) was detected with four methods: TYC dyeing, I2-KI dyeing, inorganic acid test, sucrose-based germination, the determination effects of different methods were compared, and the dynamic change of pollen viability within a day was understood. The results show that: ( 1 ) The fertile rate of pollen of A. obtusiloba is high, about 80%. Pollens diameter are 28.75 - 33.75 p,m. (2) The concentration of TYC can influence the determination of pollen vitality, when the concentration was 0.5%, it was better than the others. (3) I2-KI dyeing is not ideal, dyeing rate is only 8.33% -22.2%, and there is no significant difference within three-color flowers. (4) The pollen vitality of A. obtusiloba with three-color flowers have differences, with yellow the highest, followed by light yellow, white the minimum. TYC dyeing is a simple and rapid determination method of A. obtusiloba. Within a day, pollen vitality is maximum from 12:00 - 14:00 pro.
出处
《植物研究》
CAS
CSCD
北大核心
2013年第5期582-586,共5页
Bulletin of Botanical Research
基金
国家自然科学基金资助项目(30960066)
关键词
钝裂银莲花
测定方法
可育性
花粉
活力
Anemone obtusiloba
detection methods
fertility
pollen
viability