摘要
在构建盐胁迫下青杨microRNA文库中发现了ptc-miR801,为探索植物在盐胁迫条件下ptc-miR801参与胁迫应答的机制,本实验构建了植物表达载体pCAM2300-ami801,经根癌农杆菌EHA105介导、花序侵染法获得拟南芥转基因植株。RT-PCR半定量结果显示ptc-miR801可以在转基因拟南芥中超表达且NaCl胁迫下ptc-miRNA801转基因植株种子萌发率和根长显著高于野生型,说明ptc-miR801超表达增强了转基因拟南芥耐盐性。该试验为进一步研究miR801在杨树胁迫应答机制中的作用奠定基础。
ptc-miR801 was identified from P. cathayana by microRNA library sequence, and it had been confirmed that ptc-miRS01 was induced by salt stress. The putative targets were NAC-domain protein and Scarecrow-like gene, which were shown to play important roles in response to salt stress. To investigate the function of ptc-miR801, the artificial miR801 plant expression vector was constructed and transformed into Arabidopsis thaliana. Transformed plants were confirmed by PCR and RT-PCR. The results showed that amiRNA transcript level was markedly improved in transgenic plant compared with non-transgenic plants and the overexpression of ptc-miR801 could enhance the salt tolerance of transgenic plants. Our results would help to clarify the molecular mechanism of P. cathayana under salt stress.
出处
《植物研究》
CAS
CSCD
北大核心
2013年第5期599-604,共6页
Bulletin of Botanical Research
基金
浙江省科技厅重大专项(2010C12010)"重要生态经济树种转基因平台建立及耐盐转基因研究"
关键词
MIRNA
表达载体
拟南芥
耐盐
miRNA
expression vector
Arabidop.sis thaliana
salt stress
