重组人粒细胞集落刺激因子对脑出血大鼠神经功能的影响

Effect of recombinant human granulocyte colony-stimulating factor on neural function in rats after intracerebral hemorrhage

目的探讨重组人粒细胞集落刺激因子（rhG—CSF）对脑出血大鼠神经功能的影响。方法将48只SD大鼠按随机数字表法分为模型组和rhG-CSF治疗组，均应用大鼠脑立体定位仪注射自体不凝血法制作脑出血模型，其中rhG—CSF治疗组于造模后1h开始腹腔注射rhG-CSF[60μg／（kg·d）]，模型组注射等量生理盐水。造模后第7天及第14天对2组大鼠分别进行神经功能评分，流式细胞仪检测外周血CD34±细胞的含量，免疫组化染色观察脑出血灶周围组织微管相关蛋白2（MAP-2）的表达。结果rhG—CSF治疗组第7天及第14天的神经功能评分（0．407±0．057，0．649±0．014）、外周血CD34＋细胞含量（0．381％±0．054％，0．205％±0．088％）及MAP-2表达（0．205±0．088，0．281±0．054）均明显高于模型组（0．168±0．066，0．326±0．047；0．074％±0．028％，0．030％±0．057％；0．080±0．017，0．124±0．028）．差异有统计学意义（P〈0．05）。结论rhG—CSF可促进脑出血区神经细胞的增生和修复，对出血后脑组织具有保护作用，从而改善脑出血后神经功能。

Objective To study the effect of recombinant human granulocyte colony-stimulating factor （rhG-CSF） on neural function in rats after intracerebral hemorrhage. Methods Forty-eight SD rats were randomly divided into model group and rhG-CSF treatment group; ntracerebral hemorrhage models in the two groups were induced by rat brain stereotaxic atlas via injecting autologous incoagulable blood obtained fi：om the tail of rats. Rats in the rhG-CSF treatment group were administered with rhG-CSF at a dosage of 60 μg/ （kg. d） by intraperitoneal injection 1 h after the inducement; rats in the model group was administered with the same amount of 0.9% Nacl. On the 7^th and 14^th day of operation, the neurological severity scores of the rats in two groups were recorded and the number of peripheral blood CD34＋ cells was detected with flow cytometry; MAP-2 protein expression in the surrounding tissue of hemorrhage area at the different time points was tested by immunohistochemisty. Results In the rhG-CSF treatment group, the neurological severity scores （0.407±0.057, 0.649±0.014）, level of CD34＋ blood cells （0.381%±0.054%, 0.205%±0.088%） and MAP-2 protein expression level （0.205±0.088, 0.281 ±0.054） were significantly higher than those in the model group （0.168±0.066, 0.326±0.047; 0.074%±0.028%, 0.030%±0.057%; 0.080±0.017, 0.124±0.028, P〈0.05）. Conclusion The rhG-CSF could improve neural function of rats after intracerebral hemorrhage, which may play an important role in the process of reparation of nerve tissues and neural functional recovery after hemorrhage.