EN2与β-catenin在膀胱尿路上皮癌中的表达及其意义

Expression of Engrailed-2 and β-catenin in bladder urothelial carcinoma and their significance

目的探讨膀胱尿路上皮癌中EN2与β-catenin的表达及其意义。方法选取60例手术切除的不同病理分级和分期的膀胱尿路上皮癌组织和10例正常膀胱粘膜组织。通过免疫组织化学、Western blot及RT-PCR方法分别检测EN2与β-catenin蛋白和基因的表达情况。结果 EN2及β-catenin蛋白在膀胱尿路上皮癌中的表达明显增强(P<0.05),并与病理分级、分期的程度成正比(P<0.05);EN2及β-catenin基因在膀胱尿路上皮癌中的表达也明显增强(P<0.05),并与病理分级、分期的程度成正比(P<0.05)。结论 EN2和β-catenin在膀胱尿路上皮癌中的表达明显增强;EN2可能通过激活Wnt/β-catenin信号通路,参与膀胱尿路上皮癌的发生、发展。

Objective To investigate the expressions of Engrailed-2 （EN2） and β-catenin in bladder urothelial carcinoma and explore their significance. Methods Sixty bladder urothelial carcinoma samples of different grades and stages and 10 normal bladder mucosal tissues were examined for expressions of EN2 and β-catenin proteins and mRNA using immunochemistry, Western blotting and RT-PCR. Results Compared to normal bladder mucosa, bladder urothelial carcinoma tissues showed significantly increased expressions of EN2 and β-catenin proteins （P〈0.05）, and the high-grade carcinoma tissues exhibited significantly stronger expressions than the low-grade ones （P〈0.05）;the expressions of the proteins increased also significantly with advanced pathological stages of bladder urothelial carcinoma （P〈0.05）. The expressions of EN2 and β-catenin mRNAs showed a consistent pattern of changes with their protein expressions. Conclusion The expressions of EN2 andβ-catenin are significantly increased in bladder urothelial carcinoma. EN2 may contribute to the development and progression of bladder urothelial carcinoma by activating Wnt/β-catenin signal pathway.