NLRP3炎症小体在咪喹莫特诱导小鼠银屑病样模型中的表达及芥菜籽对其的影响

Expression of NLRP3 inflammasome in BALB/c mice with imiquimod-induced psoriasislike inflammation and therapeutic effect of mustard seed (Sinapis Alba Linn)

目的探讨NOD样受体热蛋白结构域相关蛋白3(NLRP3)炎症小体在咪喹莫特诱导的小鼠银屑病样模型中的作用及芥菜籽对NLRP3炎症小体的影响。方法将30只BALB/c小鼠随机分为3组,正常对照组、模型组(给予正常饲料并用5%咪喹莫特诱导银屑病样炎症)和实验组(给予5%芥菜籽饲料并用5%咪喹莫特诱导银屑病样炎症)。RT-PCR方法检测皮损处NLRP3、ASC、caspase-1和caspase-11的mRNA表达,免疫组化方法检测ASC和caspase-1的表达和分布,ELISA方法检测血清IL-1β和IL-18的含量。结果与正常对照组相比,小鼠银屑病样模型皮损中NLRP3、ASC、caspase-1和caspase-11的表达水平增高,血清IL-1β和IL-18的含量增高,差异有统计学意义(P<0.05);与模型组相比,芥菜籽抑制上述因子在实验组中的表达,差异有统计学意义(P<0.05)。结论 NLRP3炎症小体可能参与了银屑病样模型的形成,芥菜籽可能通过降低其表达抑制了IL-1β、IL-18引起的炎症反应而减轻银屑病损害。

Objective To investigate the role of NLRP3 inflammasome in imiquimod-induced psoriasis-like inflammation in mice and the therapeutic effects of mustard seed （Sinapis Alba Linn）. Methods Thirty BALB/c mice were randomized equally into blank control group （fed with normal forage and treated with vehicle）, model group （fed with normal forage and treated with 5% imiquimod cream）, and experimental group （fed with 5% mustard seed forage and treated with 5% imiquimod cream）. RT-PCR was used to detect the mRNA expression of NLRP3, ASC, caspase-1, and caspase-11. Immunohistochemistry was performed to determine the expression and distribution of ASC and caspase-1. ELISA was used to test the serum levels of interleukin-1β （IL-1β） and IL-18. Results Compared with the blank control group, the mice with imiquimod-induced psoriasis-like inflammation showed significantly increased NLRP3, ASC, caspase-1, and caspase-11 mRNA expressions, ASC and caspase-1 protein expressions , and serum levels of IL-1βand IL-18 （P〈0.05）. These changes were obviously attenuated by feeding the mice with mustard seed. Conclusion NLRP3 inflammasome is involved in imiquimod-induced psoriasis-like inflammation in mice, and mustard seed may suppress the inflammation induced by IL-1βand IL-18 through down-regulating the expression of NLRP3 inflammasome.