摘要
目的探讨高危型人乳头状瘤病毒(HR-HPV)检测联合hTERC(human telomerase RNA gene component)基因荧光原位杂交(FISH)检测在宫颈癌筛查诊断中的价值。方法收集深圳市深圳市龙岗中心医院妇科门诊就诊的患者和北京医院病理科2008-2010年宫颈细胞学和活检共89例,根据组织学诊断结果分为4组,采用第二代杂交捕获法(hybrid capture 2,HC-2)进行了HPV定量检测;FISH方法检测宫颈细胞hTERC基因。结果 HPV阳性率各组间比较与HPV感染载荷量各组间比较,结果相似,除CIN2-3与宫颈鳞癌组间差异无统计学意义(P>0.05)外,余各组间差异均有统计学意义(P<0.01)。hTERC基因随着CIN病变程度的增加,其阳性表达率呈现显著上升趋势,宫颈鳞癌组表达最高;具体两组间比较,对照组与CIN各组及宫颈鳞癌组间表达率差异有统计学意义(P<0.01),CIN1与CINⅡ/Ⅲ、CIN1与SCC和CINⅡ/Ⅲ与SCC组间差异无统计学意义(P>0.05)。结论 HR-HPV定量检测联合hTERC检测对于宫颈高级别上皮内病变和宫颈癌筛查的诊断具有一定的价值。
Objective To explore the predictive value of human telomerase RNA gene component （hTERC） gene amplification and high-risk human papilomavirus （HR-HPV） testing in cytologic specimens of cervix as a marker for early diagnosis of cervix carcinoma. Methods Fluorescencein situhybridization （FISH） and hybrid capture 2 （HC-2） was used to detect separately the amplification of hTERC and HR-HPV DNA of cytologic samples in 89 cases. According to histology biopsy, 89 Pap smears divided into control （normal/cervicitis （n=27））, cervical intraepithelial neoptasia （CIN I , n= 19, CIN Ⅱ/Ⅲ, n=33）, squamous carcinomas of the cervix （SCC, n=10）. Results hTERC Gene amplification of specimens was tested in 89 cases, the positive amplification rate of hTERC gene in the control group（0%）, compared to the cervical carcinomas （60%）, CINⅡ/Ⅲ （54.55%） and CIN I （36.84%）, which showed a significant difference （P〈0.001）. The rates in CIN I , CIN Ⅱ/Ⅲ and SCC were no significant difference, respectively compared to the each orther. HR-HPV DNA testing was positive in 57 cases of patients containing 11 cases of CIN I （57.89%）, 32 cases of CIN Ⅱ/Ⅲ （96.97%）, 10 cases of SCC （100%）, 4 cases of control group （14.81）. The rates in control group, CIN I , CIN Ⅱ/Ⅲ and SCC were significant difference, respectively compared to the each orther except that CIN Ⅱ/Ⅲ and SCC wsd no significant difference compared to the each orther. Conclusion The combined detection of quantitative high risk human papilloma virus and hTERC Gene is a certain value in screening cervical cancer.
出处
《解剖学研究》
CAS
2013年第4期269-272,共4页
Anatomy Research
