BDNF对哮喘小鼠C7-T5节段脊髓后角Kinesin-1表达的上调作用

The upregulative effect of BDNF on Kinesin-1 in the spinal dorsal horn of the asthmatic mice

目的探讨在哮喘发病中，脑源性神经营养因子（BDNF）对哮喘小鼠C7～T5节段脊髓后角内驱动蛋白-1（Ki．nesin-1）表达的调节作用。方法BALB／c小鼠30只，按随机数字表法均分为正常对照组、哮喘组、Anti．BDNF抗体组，利用AniRes2005肺功能仪测小鼠气道阻力、免疫荧光方法和westernblot方法检测各组小鼠cC7～T5节段脊髓后角内Kinesin-1的表达。结果哮喘组小鼠吸气阻力和呼气阻力明显高于正常组（P〈O．01），与哮喘组相比，Anti．BDNF抗体组吸气阻力和呼气阻力则明显降低；免疫荧光结果显示哮喘组C7-T5节段脊髓后角内Kinesin．1阳性产物的平均光密度值（MOD）显著高于正常对照组（P〈O．01）；而anti—BDNF抗体组与哮喘组相比，Kinesin-1阳性反应产物的MOD值则明显降低（P〈0．01）。westernblot方法也得到了相同的结论。结论BDNF被阻断后，哮喘小鼠C7～T5节段脊髓后角内Kinesin-1的表达降低。

Objective To study the regulatory effect of brain-derived neurotrophic factor （BDNF）on the expression of Ki- nesin-1 in the spinal dorsal horn of the asthmatic mice. Methods 30 BALB/c mice were randomly divided into normal control group, asthmatic group, anti-BDNF group on average. The airway resistance of mice was measured with AniRes 2005 pulmonary function meter; The expression of Kinesin-1 was observed by immunofluorescence and Western blot methods in the C7-T5 spinal dorsal horn of all the groups. Results Inspiratory resistance and expiratory resistance in the asthmatic mice were obviously higher than in normal control mice; compared with the asthmatic mice, inspiratory resistance and expiratory resistance were decreased sig- nificantly in anti-BDNF group. The mean optic density （MOD） of Kinesin-1 positive product in the C7-T5 spinal dorsal horn in- creased significantly in the asthmatic mice than in normal control by means of immunofluorescence （P〈0.01） ; but decreased in the anti-BDNF group than in the asthmatic mice （P〈0.01）, and the same result was obtain by western blot method. Conclusion The expression of Kinesin-1 in the C7-T5 spinal dorsal horn of the asthmatic mice.was decreased after BDNF was blocked.