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Rho激酶对大鼠海马神经元CRMPs mRNA表达的调节 被引量：1

CRMPs mRNA expression regulated by Rho kinase in rat hippocampal neurons
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摘要 目的探讨Rho激酶对大鼠海马神经元CRMPs mRNA表达的调节。方法体外培养新生大鼠海马神经元5d后,用Rho激酶激动剂LPA和抑制剂Y27632改变细胞内Rho激酶的活性,采用突起提取试剂盒提取神经元的突起,检测突起生长的变化,并应用实时荧光定量PCR方法检测CRMPs mRNA的表达。结果对照组细胞突起提取液吸光度值为0.0426±0.0062,用LPA处理后的细胞突起提取液吸光度值较对照组明显降低(P<0.05);Y27632组细胞突起提取液的吸光度值较对照组明显升高(P<0.05)。各基因在培养的大鼠海马神经元中均能检测到,对照组分析显示CRMP 1、2、4、5 mRNA表达水平相近且较高,CRMP3 mRNA表达水平相对较低;LPA处理后,CRMP1与CRMP3 mRNA表达水平与对照组相比明显上调(P<0.05),CRMP5 mRNA表达水平明显下调(P<0.05),CRMP2与CRMP4 mRNA表达水平与对照组相比无明显差异(P>0.05);Y27632处理后,CRMP1与CRMP3 mRNA表达水平与对照组相比明显下调(P<0.05),CRMP2、CRMP4和CRMP5 mRNA表达水平与对照组相比无明显差异(P>0.05)。结论 Rho激酶通过影响CRMP1、CRMP3和CRMP5 mRNA的表达调控神经元突起生长。 Objective To investigate the regulation of CRMPs mRNA expression by Rho kinase in cultured rat hippocampal neurons. Methods Primarily cultured neurons from hippocampus of postnatal rats were treated with Rho kinase activator LPA and inhibitor Y27632 to modulate Rho kinase＇ s activity. The neurites were extracted and the change in outgrowth of neurites was mea- sured by Neurite Outgrowth Quantification Assay Kit. The expression of CRMPs mRNA in neurons was determined by real-time quantitative PCR. Results The optical density was measured to be 0.0426＋0.0062 in the control group. When the cells were treat- ed with LPA, the absorbance was determined to be significantly lower than the control （P〈0.05）, and significantly higher when treated with Y27632 （P〈0.05）. All genes were detected in the cultured hippocampal neurons of rats. Compared with the control group, the transcript levels of CRMP1 and CRMP3 were found to be increased significantly in the LPA group （P〈0.05）, CRMP5 was found to be decreased （P〈0.05）, and CRMP2 and CRMP4 showed no change （P〉0.05）. However, in the Y27632 group CRMP1 and CRMP3 were found to be decreased significantly（P〈0.05）, and CRMP2, CRMP4 and CRMP5 showed no change （P〉 0.05） when compared with the control group. Conclusions Rho kinase modulates neurites outgrowth through regulating the expres- sion of CRMP1. CRMP3 and CRMP5 mRNA .
作者 尹义臣 张吉凤 龚晓兵 张文斌 郭国庆
机构地区 暨南大学医学院附属第四医院广州市红十字会医院神经内科 中山大学中山医学院神经生物学研究中心 暨南大学医学院解剖学系
出处 《解剖学研究》 CAS 2013年第4期296-299,304,共5页 Anatomy Research
基金 国家自然科学基金(31170941) 中央高校基本科研业务费专项资金(21612424)
关键词 RHO激酶 坍塌反应调节蛋白 实时荧光定量PCR 突起生长 Rho kinase CRMPs Real-time fluorescent quantitative RCR Neurites growth
分类号 R341 [医药卫生—基础医学]
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参考文献8

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二级参考文献14

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共引文献1

同被引文献13

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解剖学研究
2013年 第4期

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