MCP-1和FN在中耳胆脂瘤上皮中的表达和意义

Expression and the Significance of MCP-1 and FN in Middle Ear Cholesteatoma Epithelium

目的探讨单核细胞趋化因子(monocyte chemotactic factor,MCP-1)和纤维连接蛋白(fibronectin,FN)在继发性中耳胆脂瘤上皮中的表达及其对胆脂瘤上皮侵袭能力的影响。方法应用免疫组织化学MaxVisionTM法检测MCP-1和FN在30例中耳胆脂瘤上皮、20例胆脂瘤患者耳后正常皮肤、16例非胆脂瘤患者耳后正常皮肤中的表达,应用计算机图像分析系统对其阳性表达灰度值情况及分析,比较三组之间MCP-1和FN表达的差异。结果 MCP-1阳性细胞表达主要分布于胆脂瘤上皮全层,其中棘层呈高表达,MCP-1在中耳胆脂瘤上皮中阳性表达率为70%,灰度值为147.2±20.1,强于胆脂瘤患者耳后正常皮肤中的阳性表达(35%,200.8±18.4)和非胆脂瘤患者耳后正常皮肤中的阳性表达(37.5%,193.3±15.5)(P<0.05)。FN阳性细胞主要分布于胆脂瘤上皮全层,基底层、棘层和基质呈高表达,FN在中耳胆脂瘤上皮中阳性表达率为76.7%,灰度值为139.2±18.5,强于胆脂瘤患者耳后正常皮肤中的阳性表达(30%,195.0±12.9)和非胆脂瘤患者耳后正常皮肤中的阳性表达(31.3%,191.6±13.5)(P<0.05)。在30例中耳胆脂瘤上皮组织中,MCP-1和FN的灰度值与胆脂瘤的侵袭能力负相关(rmcp-1=-0.682,rfn=-0.531,P<0.01),MCP-1和FN蛋白的表达不存在线性相关。结论 MCP-1和FN均在成人中耳胆脂瘤中高表达,且与中耳胆脂瘤的侵袭能力呈负相关。

Objective To study the expression of monocyte chemotactic factor--I（MCP--1） and fibronectin （FN） in secondary acquired middle ear cholesteatoma epithelium, and to investigate the ability of cholesteatoma of erosion. Methods MaxVisionTM immunohistochemical method was used to detect the expression of MCP--1 and FN in the secondary acquired middle ear cholesteatoma tissues from 30 patients, in the retroauricular skin from 20 pa- tients and in the retroauricular skin from 16 normal subjects. Then we scanned it into a computer by an image scan- ner and quantified the gray value of them using commercial software. Results MCP-- 1 appeared to be localized in all epithelial layers of middle ear cholesteatoma, particularly in the spinous layers. The positive expression rates of MCP-1 was 70% , the gray value was 147.2±20.1, which were siginificantly higher than those of in the retroauricular skin from patients（35% ,200.8±18.4）and from normal subjects（37.5%,193.3±15.5）（P〈0.05）. The expression of FN in all epithelial layers of middle ear cholesteatoma were abundantly stained, especially in the basal and spinous layers and the matrix of cholesteatoma. The positive expression rates of FN was 76.7% , the gray value was 147.2 20.1, which were siginificantly higher than those of in the retroauricular skin from patients（30%, 195.0±12.9）and from normal subjects（31.3% ,191.6±13.5）（P〈0.05）. It showed statistically significant correlation between the expression of MCP--1 and FN and the erosion ability of middle ear cholesteatoma（rmcp-1 =-0. 682, rfn =-0. 531, P〈0.01）. There was not linear correlation between the expression of MCP--1 and FN. Oonclusion MCP--1 and FN are overexpressed in middle ear eholesteatoma. There was correlation between the expression of MCP-1 or FN and the erosion ability of middle ear eholesteatoma, indicating that MCP-1 and FN may play an im portant roles in invasive behavior of cholesteatoma.