不同炎症等级性釉质上皮型颅咽管瘤组织中炎症细胞因子的表达

Expression pattern of inflammatory cytokines at various inflammatory levels of adamantinomatous craniopharyngioma

目的 应用抗体芯片分析不同炎症等级釉质上皮型颅咽管瘤组织中炎症细胞因子表达.方法 根据邻近肿瘤正常组织交界处的炎症细胞数量对颅咽管瘤进行炎症等级评估,采用抗体芯片对不同炎症等级的颅咽管瘤炎症细胞因子进行检测,运用Western印迹实验对筛查结果进行验证.结果 重度炎症组肿瘤组织多种炎性细胞因子,包括促炎因子、趋化因子、某些细胞因子受体等的表达水平较轻度炎症组显著升高.结论 不同炎症等级的颅咽管瘤组织中炎症细胞因子的表达存在差异.

Objective To explore the expression pattern of inflammatory cytokines at various inflammatory levels of adamantinomatous craniopharyngioma by cytokine antibody array. Methods The inflammatory levels of adamantinomatous craniopharyngioma were evaluated on the basis of the number of inflammatory cells at the interface of tumor and normal tissues. And the expression Of inflammatory cytokines was examined at various inflammatory levels of adamantinomatous craniopharyngioma by inflammatory cytokine antibody array and the results were verified by Western blot. Results Compared with the mild inflammatory group, the levels of inflammatory cytokines of severe inflammatory group markedly increased, including pro-inflammatory cytokines, chemokines and cytokine receptors. Conclusion Antibody array demonstrates a significant change in cytokine profiles in adamantinomatous craniopharyngioma with severe inflammation, as compared with those with mild inflammation.