期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

循环型miR-141在胆囊癌侵袭转移和复发中的影响及其临床意义 被引量：2

Effect of circulating miR-141 on invasion and migration of gallbladder cancer cells
下载PDF
导出
摘要 目的:检测胆囊癌患者血清及肿瘤组织中microRNA的差异表达,筛选关键microRNA并阐明其与患者临床特征的相关性及其对胆囊癌细胞侵袭迁移能力的影响.方法:收集2010-09/2012-04于武汉同济医院胆胰外科进行根治性手术的胆囊癌病例12例;每例患者均采集术前外周血,术中收集手术切除肿瘤标本.同时收集同期入院的胆囊结石/健康志愿者血清14例.利用Real-time PCR对12例胆囊癌患者及14例对照血清标本中的10条microRNA表达水平进行检测;在12对胆囊癌和癌旁组织中对筛选出的差异microRNA进行验证;结合患者临床病例资料进行相关性分析.利用慢病毒载体转染,验证miR-141上调后在体外对胆囊癌GBC-SD细胞侵袭、迁移能力的影响.结果:Real-time PCR结果提示,相对于正常对照组,胆囊癌患者血清中筛选出3条差异表达循环microRNA,其中miR-141呈显著低表达,差异具有统计学意义(P<0.05);miR-141在胆囊癌组织中表达显著低于正常胆囊及癌旁组织,胆囊癌中miR-141水平较癌旁组织中低16.68倍±0.64倍,差异具有统计学意义(P<0.05);再发或转移胆囊癌患者循环miR-141表达水平较术前明显降低,差异具有统计学意义(P<0.05);miR-141上调组GBC-SD细胞侵袭细胞较阴性对照组细胞数下降10.83倍±0.37倍,迁移率下降3.48倍±0.62倍,差异具有统计学意义(P<0.01).结论:胆囊癌患者血清中miR-141表达具有差异性,miR-141在胆管癌组织中呈显著低表达,且其可在体外显著抑制胆管癌细胞侵袭迁移能力,可作为胆囊癌早期诊断及预后判断的潜在血清标志物. AIM： To screen differentially expressed microRNAs in serum and tissues of patients with gallbladder cancer and explore their effect on the migration and invasion ability of gallbladder cancer cells. METHODS： The expression of 11 microRNAs was detected in serum of 12 patients with gallbladder cancer and 14 normal controls to screen differentially expressed microRNAs. Differential expression of screened microRNAs in human gallbladder cancer tissues was verified by real-time PCR. The effect of miR-141 mimic transfection on the invasion and migration ability of human gallbladder cancer GBC-SD cells was detected by Transwell assay and wound-healing assay. RESULTS： Compared to the control group, two microRNAs （miR-141 and miR-200a） were significantly down-regulated, and one up-regulated in serum of patients with gallbladder cancer. The expression of miR-141 in human gallbladder cancer tissues was also significantly lower than that in normal gallbladder tissues （P 〈 0.05）. Expression of miR-141 in human gallbladder cancer tissues was related with distant metastasis. The invasion and migration abilities of GBC-SD cells were declined after lentivirus-mediated miR-141 up-regulation. CONCLUSION： Some microRNAs are differentially expressed in serum and tissues between patients with gallbladder cancer and healthy people. MiR-141 is probably involved in invasion and migration of gallbladder cancer.
作者 彭丰 王敏 江建新 田锐 李旭 徐盟 郭兴军 秦仁义
机构地区 华中科技大学同济医学院附属同济医院胆胰外科中心 贵阳医学院附属医院肝胆外科
出处 《世界华人消化杂志》 CAS 北大核心 2013年第25期2500-2507,共8页 World Chinese Journal of Digestology
基金 国家自然科学基金面上资助项目 Nos.81071775 81172064 81160311 81001068 81101621 湖北省自然科学重点基金资助项目 No.2011CDA030 中国博士后科学基金面上资助项目 No.2013M531983~~
关键词 胆囊癌 MICRORNA 侵袭转移 Gallbladder cancer MicroRNA Invasion and metastasis
分类号 R735.8 [医药卫生—肿瘤]
  • 相关文献

参考文献31

  • 1Jones RS. Carcinoma of the gallbladder. Surg Clin North Am 1990; 70:1419-1428 [PMID: 2247823].
  • 2Yee K, Sheppard BC, Domreis J, Blanke CD. Can- cers of the gallbladder and biliary ducts. Oncology (Williston Park) 2002; 16: 939-946, 949; discussion 949-950, 952-953, 956-957 [PMID: 12164560].
  • 3Zhai G, Yan K, Ji X, Xu W, Yang J, Xiong F, Su J, Mc- Nutt MA, Yang H. LAPTM4B allele *2 is a markerof poor prognosis for gallbladder carcinoma. PLoS One 2012; 7:e45290 [PMID: 22984631 DOI: 10.1371/ journal.pone.0045290].
  • 4Liu C, Sun B,.An N, Tan W, Cao L, Luo X, Yu Y, Feng F, Li B, Wu M, Su C, Jiang X. Inhibitory effect of Survivin promoter-regulated oncolytic adenovi- rus carrying P53 gene against gallbladder cancer. Mol Oncol 2011; 5:545-554 [PMID: 22032823 DOI: 10.1016/j.molonc.2011.10.001].
  • 5Baer C, Claus R, Plass C. Genome-wide epigenetic regulation of miRNAs in cancer. Cancer Res 2013; 73: 473-477 [PMID: 23316035 DOI: 10.1158/0008-5472. CAN-12-3731].
  • 6DeSano JT, Xu L. MicroRNA regulation of cancer stem cells and therapeutic implications. AAPS J 2009; 11:682-692 [PMID: 19842044 DOI: 10.1208/ s12248-009-9147-7].
  • 7He J, Zhang W, Zhou Q, Zhao T, Song Y, Chai L, Li Y. Low-expression of microRNA-107 inhibits cell apoptosis in glioma by upregulation of SALL4. Int ] Biochem Cell Biol 2013; 45:1962-1973 [PMID: 23811124 DOI: 10.1016/j.biocel.2013.06.008].
  • 8Olivieri F, Rippo MR, Monsurr6 V, Salvioli S, Capri M, Procopio AD, Franceschi C. MicroRNAs linking inflamm-aging, cellular senescence and cancer. Age- ing Res Rev 2013 May 17. [Epub ahead of print] [PMID: 23688930 DOI: 10.1016/j.arr.2013.05.001].
  • 9Eichelser C, Flesch-Janys D, Chang-Claude J, Pantel K, Schwarzenbach H. Deregulated Serum Con- centrations of Circulating Cell-Free MicroRNAs miR-17, miR-34a, miR-155, and miR-373 in Hu- man Breast Cancer Development and Progression. Clin Chem 2013 Jun 7. [Epub ahead of print] [PMID: 23748853 DOI: 10.1373/clinchem.2013.205161].
  • 10Li A, Yu J, Kim H, Wolfgang CL, Canto ML Hru- ban RH, Goggins M. MicroRNA Array Analysis Finds Elevated Serum miR-1290 Accurately Distin- guishes Patients with Low-Stage Pancreatic Cancer from Healthy and Disease Controls. Clin Cancer Res 2013; 19:3600-3610 [PMID: 23697990 DOI: 10.1158/1078-0432.CCR-12-3092[.

二级参考文献35

  • 1Dillhoff M, Liu J, Frankel W, Croce C, Bloomston M. MicroRNA-21 is overexpressed in pancreatic cancer and a potential predictor of survival. J Gastrointest Surg 2008; 12: 2171-2176.
  • 2Schmiegel WH, Kreiker C, Eberl W, Arndt R, Classen M, Greten H, Jessen K, Kalthoff H, Soehendra N, Thiele HG. Monoclonal antibody defines CA 19-9 in pancreatic juices and sera. Gut 1985; 26: 456-460.
  • 3Kuhlmann KF, de Castro SM, Wesseling JG, ten Kate FJ, Offerhaus GJ, Busch OR, van Gulik TM, Obertop H, Gouma DJ. Surgical treatment of pancreatic adenocarcinoma; actual survival and prognostic factors in 343 patients. Eur J Cancer 2004; 40: 549-558.
  • 4Slesak B, Harlozinska-Szmyrka A, Knast W, Sedlaczek P, van Dalen A, Einarsson R. Tissue polypeptide specific antigen (TPS), a marker for differentiation between pancreatic carcinoma and chronic pancreatitis. A comparative study with CA 19-9. Cancer 2000; 89: 83-88.
  • 5Ni XG, Bai XF, Mao YL, Shao YF, Wu JX, Shan Y, Wang CF, Wang J, Tian YT, Liu Q, Xu DK, Zhao P. The clinical value of serum CEA, CA19-9, and CA242 in the diagnosis and prognosis of pancreatic cancer. Eur J Surg Oncol 2005; 31: 164-169.
  • 6Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell 2004; 116: 281-297.
  • 7Slack FJ, Weidhaas JB. MicroRNA in cancer prognosis. N Engl J Med 2008; 359: 2720-2722.
  • 8Visone R, Croce CM. MiRNAs and cancer. Am J Pathol 2009; 174: 1131-1138.
  • 9Mirnezami AH, Pickard K, Zhang L, Primrose JN, Packham G. MicroRNAs: key players in carcinogenesis and novel therapeutic targets. Eur J Surg Oncol 2009; 35: 339-347.
  • 10Calin GA, Dumitru CD, Shimizu M, Bichi R, Zupo S, Noch E, Aldler H, Rattan S, Keating M, Rai K, Rassenti L, Kipps T, Negrini M, Bullrich F, Croce CM. Frequent deletions and down-regulation of micro- RNA genes miR15 and miR16 at 13q14 in chronic lymphocytic leukemia. Proc Natl Acad Sci U S A 2002; 99: 15524-15529.

共引文献10

同被引文献43

  • 1赫捷,陈万青.2012中国肿瘤登记年报[M].北京:军事医学科学出版社,2012:12-25.
  • 2Yee K,Sheppard BC,Domreis J,et al.Cancers of the gallbladder and biliary ducts[J].Oncology(Williston Park),2002,16(7):939-946.
  • 3Bartel DP.MicroRNAs; genomics,biogenesis,mechanism,and function[J].Cell,2004,116(2):281-297.
  • 4Ruby JG,Jan CH,Bartel DP.Intronic microRNA precursors that bypass Drosha processing[J].Nature,2007,448(7149);83-86.
  • 5Yi R,Qin Y,Macara IG,et al.Exportin-5 mediates the nuclear export of pre-microRNAs and short hairpin RNAs[J].Genes Dev, 2003,17(24):3011-3016.
  • 6Okamura K,Hagen JW,Duan H,et al.The mirtron pathway gen- erates microRNA-class regulatory RNAs in Drosophila[J].Cell, 2007,130(1):89-100.
  • 7Calin GA,Sevignani C,Dumitru CD,et al.Human microRNA genes are frequently located at fragile sites and genomic regions in- volved in cancers[J].Proc Natl Acad Sci U S A,2004,101(9):2999-3004.
  • 8He L,Hannon GJ.MicroRNAs:small RNAs with a big role in gene regulation[J].Nat Rev Genet,2004,5(7):522-531.
  • 9Poy MN,Eliasson L,Krutzfeldt J,et al.A pancreatic islet-specific microRNA regulates insulin secretion[J].Nature,2004,432(7014):226-230.
  • 10Zhang B,Pan X,Cobb GP,et al.microRNAs as oncogenes and tumor suppressors[J].Dev Biol,2007,302(1):1-12.

引证文献2

二级引证文献14

世界华人消化杂志
2013年 第25期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部