艾灸对Helicobacter pylori胃炎大鼠单核细胞NF-κB、IκBα含量的影响

Effect of moxibustion treatment on expression of NF-κB and Iκ Bα in peripheral blood monocytes of rats with Helicobacter pylori-associated gastritis

目的:探讨艾灸对幽门螺杆菌(Helicobacter pylori,H.pylori)胃炎大鼠胃黏膜HE染色镜检胃黏膜炎症积分值和单核细胞核因子-B(nuclear factor-B,NF-B)、I B含量的影响,初步揭示艾灸干预H.pylori胃黏膜炎性损伤,保护胃黏膜的机制.方法:50只健康大鼠随机分为5组,即A空白组、B模型组、C艾灸组、D艾灸非穴点组和E电针组,每组10只.采用H.pylori灌胃造模,蛋白免疫印记法检测大鼠外周血单核细胞NFB、I B的含量.结果:与A组比较,B组大鼠胃黏膜HE染色镜检胃黏膜炎症积分值和单核细胞NF-B含量显著升高(0±2.0 vs 2.5±2.5,0.54±0.11/-actinvs 0.36±0.13/-actin,P<0.01),I B含量显著降低(0.21±0.03/-actin vs 0.65±0.18/-actin,P<0.01);与B组比较,C组大鼠胃黏膜H E染色镜检胃黏膜炎症积分值和单核细胞NF-B含量显著减低(2.5±2.5 vs 0±2.00,0.36±0.13/-actin vs 0.50±0.04/-actin,P<0.01),I B含量显著升高(0.65±0.18/-actin vs 0.24±0.06/-actin,P<0.01);与D组相比,C组大鼠胃黏膜HE染色镜检胃黏膜炎症积分值和单核细胞NF-B含量明显升高(3.00±2.5 vs 0±2.00,0.36±0.12/-actin vs 0.50±0.04/-actin,P<0.01),I B含量显著降低(0.64±0.19/-actin vs 0.24±0.06/-actin,P<0.01);与E组相比,C组大鼠胃黏膜HE染色镜检胃黏膜炎症积分值和单核细胞NF-B含量明显升高(3±2.75 vs 0±2.00,0.35±0.10/-actin vs 0.50±0.04/-actin,P<0.01),I B含量显著降低(0.52±0.17/-actinvs 0.24±0.06/-actin,P<0.01).结论:艾灸穴位可减轻H.pylori胃炎胃黏膜炎性损伤,此作用可能与艾灸诱导单核细胞I B大量表达,抑制NF-B表达,减少炎性细胞因子的释放,减轻胃黏膜炎症损伤有关.

AIM： To explore the effect of moxibustion treatment on gastric inflammatory injury and expression of NF-κB and IκBα in peripheral blood monocytes of rats with Helicobacter pylori （H. pylori）-associated gastritis, to reveal the mechanisms underlying the protective effect of moxibustion treatment against gastric inflammatory injury. METHODS： Fifty healthy rats were randomly divided into five groups, namely, a control group （A）, a H. pylori model group （B）, a moxibustion at acupoints group （C）, a moxibustion at non-acupoints group （D） and an electro-acupuncture group （E）. Gastritis was induced by oral gavage with live H. pylori. The expression of NF-κB and IκBα in peripheral blood monocytes was detected by Western blot. RESULTS： Compared to group A, gastric mucosal inflammation score and expression of NF-κB in monocytes were significantly increased （0 ± 2.0 vs 2.5 ± 2.5, 0.54 ± 0.11/β-actin vs 0.36 ± 0.13/β-actin, both P 〈 0.01）, and expression of IκBα in monocytes was significantly decreased in group B （0.21 ± 0.03/β-actin vs 0.65 ± 0.18/β-actin, P 〈 0.01）. Compared to group B, gastric mucosal inflammation score and expression of NF-κB in monocytes were significantly decreased （2.5 ± 2.5 vs 0 ± 2.00, 0.36 ± 0.13/β-actin vs 0.50 ± 0.04/β-actin, both P 〈 0.01）, and expression of IκBα in monocytes was significantly increased in group C （0.65 ± 0.18/β-actin vs 0.24 ± 0.06/β-actin, P 〈 0.01）. Compared to group D, gastric mucosal inflammation score and expression of NF-κB in monocytes were significantly decreased （3.00 ± 2.5 vs 0 ± 2.00, 0.36 ± 0.12/β-actin vs 0.50 ± 0.04/β-actin, both P 〈 0.01）, and expression of IκBα in monocytes was significantly increased in group C （0.64 ± 0.19/β-actin vs 0.24 ± 0.06/β-actin, P 〈 0.01）. Compared to group E, gastric mucosal inflammation score and expression of NF-κB in monocytes were significantly decreased （3 ± 2.75 vs 0 ± 2.00, 0.35 ± 0.10/β-actin vs 0.50 ± 0.04/β-actin, both P 〈 0.01）, and expression of IκBα in monocytes was significantly increased in group C （0.52 ± 0.17/β-actin vs 0.24 ± 0.06/β-actin, P 〈 0.01）. CONCLUSION： Moxibustion at acupoints can reduce H. pylori-induced gastric mucosal inflammatory injury possibly via mechanisms associated with reducing NF-κB expression in monocytes and decreasing the release of inflammatory cytokines.