2型糖尿病患者骨密度改变与血浆klotho水平及其相关因素的关系

The Correlation of Bone Mineral Density with Plasma Klotho Levels and Its Related Factors in Type 2 Diabetic Patients

目的研究2型糖尿病(T2DM)患者骨密度改变与血浆klotho(KL)水平及其相关因素的关系.方法采用双能X线骨密度仪对159例T2DM患者进行骨密度测定,分为骨量正常、骨量减少和骨质疏松3组,用双抗体夹心酶联免疫吸附法(ELISA)测定空腹血浆KL水平,并检测相关临床资料和生化指标,比较分析各组间的差异及其相关因素.结果 (1)3组人群KL水平无明显差异[(4.95±0.48 vs 4.96±0.47 vs 4.91±0.49,P>0.05];(2)年龄、DM病程、HDL-C和BMI是影响T2DM患者骨密度改变的独立相关因素(B分别为0.037、0.005、-1.011、-0.175,OR分别为1.038、1.005、0.364、0.84).结论 T2DM患者骨密度下降及骨质疏松可能与血浆KL水平无相关性,而年龄和DM病程是其危险因素,HDL-C和BMI是其保护因素.

Objective To investigate the correlation of bone mineral density （BMD） with plasma Klotho levels and its related factors in type 2 diabetic patients （T2DM） . Methods BMD was measured by Dual-energy X-ray absorptiometry （DEXA） in 159 T2DM patients. The patients were divided into three groups： normal bone mass, reduced bone mass and osteoporosis. The fasting plasma levels of Klotho were detected in these patients using enzyme linked immuno sorbent assay （ELISA） , clinical and biochemical parameters also were tested, the difference and related factors were compared and analyzed in each group. Results Plasma Klotho levels were not significantly different among the three groups （4.95 ± 0.48 vs 4.96 ± 0.47 vs 4.91 ± 0.49, P〉0.05） . BMD at the first, second, third, fourth and total lumbar spine, femoral neck, trochanter and total body were not associated with plasma Klotho levels in these patients （P 〉0.05） . Age, diabetic duration, HDL-C and BMI were independent determinants for BMD in T2DM patients. Conclusions BMD might be not associated with plasma Klotho level in T2DM patients. But age, diabetic duration, HDL-C and BMI are associated with reduced BMD and osteoporosis in T2DM patients.