摘要
目的探讨姜黄素（Cur）对AD细胞活力及HMGBl表达的影响。方法对数期生长的PCI2细胞分为5组：空白组（A组）不做处理，Aβ25-35模型组（B组）加入20Ixmol／L的Aβ25-35，Aβ25-355＋Cur治疗组（c组）加入20μmol／L的Aβ25-355和1μmoL／L的Cur，Aβ25-35＋rHMGl损伤组（D组）加入20μmol／L的AB25。5和500ng／ml的HMGBl，Aβ25-35＋溶剂对照组（E组）加入20μmol／L的Aβ25-35和1ul／ml的DMSO，孵育24h后行细胞形态学观察、免疫荧光定性和免疫蛋白印迹（Western blot）法检测HMGBl蛋白的表达情况。结果与A组相比，B、D、E组细胞活力明显下降（0．76±0．06、0．63±0．02、0．75±0．03比1．22±0．06，P〈0．05）、胞内HMGBl表达明显升高（1．19±0．14、1．12±0．16、1．16±0．09比0．85±0．04，P〈0．05）；与B组相比，C组细胞活力上升33％、HMGB1表达下降31％（P〈0．05）。B组较A组存在大量的HMGBl核外释放，而c组HMGBl的核外释放较B组减少。结论姜黄素可减轻Aβ25-35引起的PCI2细胞毒性，其机制与下调HMGB1的表达、抑制HMGB1的核外释放有关。
Objective To explore the effects of curcumin on the expression of high mobility group boxl （ HMGB1 ） , cell viability and morphology in a cellular model of Alzheimer＇s disease （AD）. Methods Cultured PC12 cells in logarithmic growth phase were divided into 5 groups： normal cell group （A, non- treatment）, model control group （B, 20 μmoL/L Aβ25-35）, curcumin treatment group （C, 20 μmol/LAβ25-35 ＋ 1 p.mol/L Cur） , Aβ25-35 ＋ rHMG1 （ D, 20 μmol/L Aβ25-35 ＋ 500 ng/ml HMGB1 ） and solvent control group （ E, 20 μmol/L Aβ25-35 ＋ 1 p,1/ml DMSO）. Cell viability was examined by methyl thiazolyl tetrazolium （M＇IT）. And the cellular expression and distribution of HMGB1 were detected by immunofluorescence and Western blot 24 hours later. Results Compared with group A, the levels of cell viability in groups B, D and E significantly declined （ 0. 76 ±0. 06, 0. 63 ± 0. 02, 0. 75±0.03 vs 1.22±0. 06, P 〈 0. 05） while the expression of HMGB1 increased （1.19±0. 14, 1.12 ±0. 16, 1.16 ± 0. 09 vs 0. 85 -0. 04, P 〈0. 05）. Compared with group B, cell viability in group C significantly increased by 33% （ 1.01±0.05, P 〈0. 05） while the expression of HMGB1 declined by 31% （0. 78±0. 03, P 〈0.05）. A larger amount of extracellular HMGB1 was released in group B compared with group A. And the extracellular release of HMGB1 declined less in group C versus group B. Conclusion Curcumin may reduce Aβ25-35- induced cytotoxicity through a down-regulated expression of HMGB1 and an inhibition of extracellular release of HMGB1 in PC12 cell.
出处
《中华医学杂志》
CAS
CSCD
北大核心
2013年第35期2826-2829,共4页
National Medical Journal of China
基金
基金项目：国家自然科学基金（81271204）
浙江省自然科学基金（Y2100231）
