葡萄糖调节蛋白58在非小细胞肺癌组织中的表达及其临床意义

Expression and clinical significance of GRp58 in non-small cell lung cancer

目的:探讨葡萄糖调节蛋白58(glucose-regulated protein 58,GRp58)GRp58在非小细胞肺癌组织中的表达情况及其与肺癌临床病理特征间的关系。方法:采用蛋白印迹法及免疫组化法检测40例非小细胞肺癌组织及配对癌旁组织中GRp58的表达及定位情况,并分析GRp58表达量与肺癌临床病理特征间的关系。结果:GRp58主要存在于非小细胞肺癌的细胞胞质中,非小细胞肺癌组织中GRp58的表达量(0.635±0.361)低于配对癌旁组织(0.899±0.245),差异有统计学意义(P<0.05),且GRp58的表达量与非小细胞肺癌临床分期有关(P<0.05),而与患者的年龄、性别、病理类型、组织学分化程度、淋巴结转移及吸烟情况均无关(P>0.05)。结论:GRp58表达量与非小细胞肺癌的临床分期有关,其低表达与非小细胞肺癌晚期可能相关。

Objective： To investigate the expression of glucose-regulated protein 58 （GRp58） and the correlation between CRp58 expression level and clinicopathologic features in non-small cell lung cancer. Methods： The expression and location of GRp58 were detected in 40 matched non-small cell lung cancer tissue and adjacent non-cancerous tissue by Western blot and immunohistochemistry. The association between the clinicopathologic features and GRp58 expression was analyzed. Results： The positive spots were mainly found in the cytoplasm of non-small cell lung cancer cell. The expression level of GRp58 in non-small cell lung cancer tissue was lower than that in matched adjacent non-cancerous tissue （0.635±0.361 vs 0.899±0.245） （P〈0.05）. The expression level of GRp58 in non-small cell lung cancer tissue was correlated with the clinical stage of cancer （P〈0.05）,and has no correlation with gender, age, pathological typing, degree of differentiation, lymph node metastasis and smoking （P〉0.05）. Conclusions： GRp58 is down-regulated in non-small cell lung cancer tissue as compared with that in adjacent non-cancerous tissue. Lower GRp58 expression in non-small cell lung cancer might have a correlation with the progress of cancer.