实时荧光聚合酶链反应检测性病患者泌尿生殖道沙眼衣原体的临床分析

Clinical analysis of Chlamydia trachomatis urogenital tract infection in patients with sexually transmitted diseases via real time fluorescent polymerase chain reaction

采用实时荧光聚合酶链反应(PCR)检测性病患者泌尿生殖道沙眼衣原体,进行临床和实验室分析。采集380例男女性病患者泌尿生殖道分泌物,进行多形核白细胞数检测和荧光PCR。结果显示,216例男性患者中,尿道多形核白细胞数≥5个者占92.59%,荧光PCR检测沙眼衣原体阳性62例,阳性率为28.70%;沙眼衣原体合并淋病奈瑟菌感染30例,合并感染率为13.89%。164例女性患者中,87例宫颈管内多形核白细胞数≥10个(占53.05%),荧光PCR检测沙眼衣原体阳性33例(占20.12%)。结果提示,性病门诊开展实时荧光PCR检测沙眼衣原体可提高检测阳性率和控制沙眼衣原体传播。

380 urogenital tract secretion samples from patients with sexually transmitted diseases were subjected for Chlamydia trachomatis detection by polymorphonuclear leukocyte count and real-time fluorescent polymerase chain reaction （PCR）. The results showed that of 216 male patients 92.59% presented urethral polymorphonuclear leukocyte count 5, and 62 cases （28.70%） were Chlamydia trachomatis-positive detected by PCR. There were 30 cases with Chlamydia trachomatis and Neisseria gonorrhoeae coinfection （13.89%）. Of 164 female patients, 53.05% presented cervical canal polymorphonuclear leukocyte count 910, and 33 cases （20.12%） were Chlamydia trachomatis-positive detected by PCR. The results indicated that the application of real-time fluorescent PCR based assay can improve the clinical management of Chlarnydia trachomatis.