补阳还五汤对沙鼠脑缺血损伤细胞凋亡的影响及机理研究

Effect of Buyang Huanwu Decoction on Neural Cell Apoptosis After Cerebral Ischemia in Gerbils and Its Mechanism

目的 :研究补阳还五汤对沙鼠脑缺血损伤后神经细胞凋亡的作用及可能的作用机理。方法 :采用沙土鼠双侧颈总动脉夹闭脑缺血再灌注模型 ,于再灌后 12 0h取大脑半球 ,在解剖显微镜下切取海马 ,电镜观察神经细胞凋亡的情况 ;同样模型 ,于缺血再灌注后 4 8h取脑组织测定Na+、K+-ATP酶、Ca2 +-ATP酶、Mg2 +-ATP酶活性、乳酸含量、NO含量、一氧化氮合酶 (NOS)活性、氨基酸含量等。结果 :补阳还五汤可使发生海马CA1区神经细胞凋亡的例数明显减少 ;可防止缺血再灌注后脑组织Na+、K+-ATP酶和Ca2 +-ATP酶活性的降低 ;增加NO含量 ,提高NOS活性 ;可使缺血再灌注 4 8h后脑内谷氨酸 (Glu)含量降低。结论 :补阳还五汤可对抗沙鼠脑缺血再灌注损伤后的神经细胞凋亡 ,其作用机理与改善脑能量代谢、调节NO的合成。

To study the effect of Buyang Huanwu Decoction (BHD) on neural cell apoptosis after cerebral ischemia in gerbils and its mechanism, gerbil model with cerebral ischemia was established by clamping of bilateral carotid arteries and then reperfusion. Neural cell apoptosis of hippocampal gyrus slides was observed under electron microscope 120 hours after reperfusion. The activities of Na + -K + ATPase , Ca 2+ ATPase , Mg 2+ ATPase and NOS, and the contents of lactic acid, NO and amino acid in cerebral tissues were detected 120 hours after reperfusion. The results showed that BHD can reduce the incidence of neural cell apoptosis of hippocampal gyrus, prevent the decrease of Na + -K + ATPase and Ca 2+ ATPase activities, increase NO content and NOS activity, and reduce cerebral glucose content. It is indicated that BHD can counteract neural cell apoptosis after cerebral ischemia in gerbils and its mechanism may be related to the improvement of cerebral energy metabolism, regulation of NO synthesis and antagonism of toxic effect of excitatory amino acid.