巴戟天离体培养及植株再生的研究

In-Vitro Culture and Plant Regeneration of Morinda officinalis How

以巴戟天无菌苗的茎尖、带节茎、无节茎为材料进行离体培养。结果表明 :外植体表面消毒采用70 %乙醇预处理 30s ,再用 0 .1%的升汞浸泡 10～ 15min ,效果较好。茎尖和带节茎培养 ,于含有 6-苄基氨基嘌呤 ( 6-benzylaminopurine。BA)的MurashigeandTucker (MT)培养基上 ,均能直接出芽 ,带节茎出芽率最高 ,为 97.5%。适宜的BA浓度为 1mg·L- 1,BA浓度升高 ,出芽率下降。根的诱导 ,以MT加 0 .2～0 .5mg·L- 1的萘乙酸 (naphthleneaceticacid ,NAA)培养基较好 ,生根率可达 80 .0

The sterile stem tips, nodular and non-nodular stem of Morinda officinalis How were cultured in vitro. The results showed that the most suitable procedure for sterilization of explants was soaking into 0.1% HgCl 2 solution for 10 to 15 minutes after pretreating with 70% ethanol for 30 seconds. MT culture medium with BA was effective to induce direct shooting of stem tips and nodular stem, the shooting rate of nodular stem being 97.5% . The optimal BA concentration was 1 mg·L -1 , the shooting rate would decrease when the concentration of BA increased. As for the induction of rooting, MT culture medium adding with 0.2 to 0.5 mg·L -1 NAA was optimal, the rooting rate being over 80.0% .