涎腺腺样囊性癌细胞转移潜能与细胞外基质的关系

The different metastatic potential of salivary adenoid cystic carcinonma and extracellular matrix

目的 了解不同转移能力的涎腺腺样囊性癌 (salivaryadenoidcysticcarcinoma ,SACC)细胞与细胞外基质粘附、对细胞外基质侵袭及趋化运动的能力。方法 MTT法测定SACC -LM ,SACC - 83,ACC -M ,ACC - 2与纤维粘连蛋白、层粘连蛋白、Ⅳ型胶原的粘附 ,用改良Boydenchamber方法观察了SACC细胞对人工重组基底膜的侵袭以及趋化运动。结果 高转移的SACC -LM与细胞外基质的粘附低于SACC - 83,而ACC -M与细胞外基质的粘附高于ACC - 2。SACC与纤维粘连蛋白的粘附高于和层粘连蛋白、Ⅳ型胶原的粘附。SACC -LM ,ACC -M的侵袭和运动能力强于SACC - 83,ACC - 2。结论 不同转移能力的SACC细胞与细胞外基质的粘附能力不同。高转移潜能的涎腺腺样囊性癌细胞的侵袭能力。

Objective To investigate the relation between metastatic potential of salivary cystic carcinoma (SACC) and extracellular matrix (ECM).Methods Highly metastatic SACC cell lines SACC-LM,ACC-M and lowly metastatic cell lines SACC-83,ACC-2 were adopted.Tumor cell-ECM adhesion was assayed by MTT technique.Invasion and migration of SACC were evaluated using modified Boyden chamber.Results Tumor cell-ECM adhesion was observed less in SACC-LM than in SACC-83,whereas more adhesion in ACC-M than in ACC-2.In addition,both SACC-LM and ACC-M had higher invasion and migration.Conclusion The SACC cells with different metastatic potential exhibit varied adhesion to ECM.It is suggested that highly metastatic SACC cells have higher invasion and migration.