纹状体源性神经营养因子治疗帕金森病大鼠模型的实验研究

An Experimental Study of Striatal-derived Neurotrophic Factor Treating the Rat Model of Parkinson′s Disease

【目的】探讨纹状体源性神经营养因子 (SDNF)在帕金森病 (PD)治疗方面的应用前景。【方法】建立 6 羟基多巴胺 (6 OHDA)诱导的大鼠“半”PD模型 ,进行早期一次性注射SDNF ,以及损伤两周后重复注射SDNF ,以观察SDNF对模型鼠的治疗作用。【结果】早期一次性注射SDNF能明显阻止中脑黑质多巴胺 (DA)神经元数量的减少。而损伤两周后重复注射SDNF ,虽不再能改变DA神经元的数量 ,但能降低模型鼠由阿朴吗啡 (APO)诱导的旋转 ,以及增加损伤侧纹状体内DA含量和降低HVA(高香草酸 ) /DA值。【结论】SDNF对 6 OHDA诱导的中脑DA神经元的变性有阻止作用 ,同时对残留DA神经元的功能有增强作用。

Objective To explore the therapeutic potential of striatal-derived neurotrophic factor (SDNF) in Parkinson′s disease. Method The 6-hydroxydopamine (6-OHDA) induced hemiparkinsonian rat model was established and the intranigral single injection of SDNF at early stage of 6-OHDA lesion and the supernigral repeated injection of SDNF 2 weeks later in 6-OHDA lesion were performed to observe the therapeutic role of SDNF on the rat model. Results The early single injection of SDNF could arrest a decline of the number of mesencephalic dopamine (DA) neurons caused by 6-OHDA lesion.Although there was no obvious influence on the survival number of mesencephalic DA neurons in the repeated injection of SDNF 2 weeks later in 6-OHDA lesion,it could decrease the average rate of apomorphine-induced rotations of rat model and increase the level of DA as well as reduce the rate of homovanillic acid (HVA) level to DA level in the lesion side of striatum of rat model. Conclusion SDNF may prevent the degeneration of mesencephalic DA neurons at early stage of 6-OHDA lesion and enhance the function of remaining DA neurons later.