转移力不同的肺癌克隆细胞中nm23-H1基因产物NDPK的表达

The Expression of nm23-H1 Gene Protein NDPK in Human Lung Carcinoma Cloning Cells with Different Metastatic Potential

【目的】比较转移抑制基因nm2 3 H1表达产物核苷酸二磷酸激酶 (nucleosidediphosphatekinase ,NDPK)在转移力不同的肺癌克隆株中的表达。【方法】通过单细胞克隆技术及裸鼠接种筛选出高转移及低转移的人肺巨细胞癌克隆细胞株及高转移的人肺腺癌克隆细胞株 ,用LSAB免疫组化法检测nm2 3 H1基因产物NDPK的表达。【结果】NDPK在高转移及低转移的人肺巨细胞癌克隆细胞移植瘤及转移瘤中均有表达 ,高转移株的阳性细胞数目及强度均高于低转移株和裸鼠正常肺组织 ;NDPK在高转移的肺腺癌克隆细胞移植瘤中有表达 ,表达强度低于裸鼠正常肺组织。【结论】nm2 3 H1基因对肺巨细胞癌并不具转移抑制基因的功能 ,而可能促进肿瘤的发生、发展和转移。

Objective To compare the expression of nucleoside diphosphate kinase(NDPK),the protein of metastasis suppressor gene nm23-H1 in clonal cell subpopulations with different metastasis from human lung carcinoma. Methods Four sub- lines with different metastatic potential were isolated from human lung giant cell carcinoma and adenocarcinoma cell lines by the single cell cloning technique and inoculated into nude mice. NDPK in the neoplastic cells of the transplanted tumors and metastatic tumors of the nude mice was detected by LSAB. Results The expression of NDPK can be detected in clonal cell subpopulations from human lung large cell carcinoma, the higher metastatic potential,the stronger expression. The expression of NDPK can also be detected in human lung adenocarcinoma with high metastatic potential, but is lower than the normal lung tissue of the nude mice. Conclusions nm23-H1 is not a metastasis suppressor gene in lung large cell carcinoma, and may be associated with its development, progression and metastasis.