两种体色生物型桃蚜对杀虫剂敏感性差异及其与酶活力的关系

The sensitivity of two body colour bio-types of the green peach aphid Myzus persicae (Sulzer) (Hemiptera: Aphididae) to the pesticides and its correlation to enzyme activity

为了明确不同体色生物型桃蚜Myzus persicae(Suizer)对药剂的敏感性差异,本文采用室内生物测定和酶活力测定法检测不同体色生物型桃蚜对杀虫剂的敏感性以及与3种解毒酶和1种靶标酶活力关系。结果表明,红色生物型的敏感性明显低于绿色生物型,其中对吡虫啉的敏感性差异最大,LC50分别为679.6648和28.4597mg/L,红色为绿色生物型23.88倍;其次为毒死蜱,LC50分别为1229.5798和193.6816mg/L,红色为绿色生物型6.35倍;两种生物型桃蚜对阿维菌素敏感性差异最小,红、绿色生物型的LC50分别为31.1678和29.0966mg/L,红色为绿色生物型1.07倍。红色生物型体内3种解毒酶(羧酸酯酶、谷胱甘肽-S-转移酶和多功能氧化酶P450)的比活力均高于绿色生物型,红色型羧酸酯酶比活力为绿色型的3.1倍,谷胱甘肽-S-转移酶为4.1倍,多功能氧化酶P450为1.5倍,两种体色生物型3种解毒酶的活力差异均达显著水平。两种体色生物型体内乙酰胆碱酯酶比活力没有差异,说明乙酰胆碱酯酶比活力与敏感性关系不大。

In order to survey the difference of sensitivity to insecticides between the two body colour bio - types of the green peach aphid Myzus persicae （Sulzer） （ Hemiptera ： Aphididae） , the bioassay was conducted in the laboratory, and three detoxifying enzymes and acetylcholinesterase activities of the two colour bio- types were tested. The results showed that the green bio -type was more sensitive to the insecticides than the red bio - type. There was a significant difference in the sensitivity to the imidacloprid between the red bio - type and green bio - type. The LCso of red bio - type was 23.88 times higher than that of the green bio - type with the value was 679.6648 and 28. 4597 rag/L, respectively. The difference of the sensitivity to chlorpyrifos between two body colour bio - types was moderate. The LCs0 of red bio - type was 6. 35 times higher than that of the green bio - type with the value was 1229. 5798 and 193.6816 mg/L, respectively. There was a little difference in the sensitivity to the abamectin between the two body colour bio - types. The LCso of red bio - type was 1.07 times as much as that of the green bio -type with the value was 31. 1678 and 29. 0966 mg/L, respectively. The esterase, glutathione -S - transferase and mixed function oxidase specific activity of red bio - type was extraordinarily higher than that of the green bio -type. The esterase, glutathione -S- transferase and mixed function oxidase specific activity of the red bio - type were 3.1, 4. 1 and 1.5 times as much as that of the green bio - type, respectively, whereas there was no difference in the acetylcholinesterase specific activity between the two body colour bio - types of M. persicae, indicating that the acetylcholinesterase specific activity was not correlate with the sensitivity.to the insecticides.